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Figure 2 | Microbial Cell Factories

Figure 2

From: High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

Figure 2

Cell growth. Cell density by OD600 readings for EnBase (A) as well as TB and ZYM-5052 (B) cultures of E. coli RB791[pQE30:adh] in Ultra Yield and Erlenmeyer flasks. One unit of OD600 corresponds to dry cell weight of 0.27 g l-1. Time of inducer addition is indicated by a straight vertical line. In EnBase cultures the Booster tablet was added at the same time with inducer. Note the different scales in figures A and B. EB: EnBase; TB: Terrific Broth; ZYM: ZYM-5052 autoinduction medium; UY: Ultra Yield Flask; Erl: Erlenmeyer flask. Concentrations of glucose-releasing enzyme in EnBase cultures (A) are presented in the legend in U l-1.

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Microbial Cell Factories

ISSN: 1475-2859

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