Substrate and pH | enzyme | Km (mM) | kcat (s-1) | kcat/Km (M-1 s-1) |
---|---|---|---|---|
Glucose, pH = 5.5 | wt | 10.2 ± 0.2 | 180 ± 3 | 17.6 × 103 |
 | rec | 10.1 ± 0.4 | 179 ± 4 | 17.7 × 103 |
Glucose, pH = 7.5 | wt | 19.0 ± 0.3 | 380 ± 6 | 20.0 × 103 |
 | rec | 17.1 ± 0.7 | 418 ± 4 | 24.5 × 103 |
Xylose, pH = 5.5 | wt | 21 ± 0.6 | 40 ± 1.5 | 1.90 × 103 |
 | rec | 26 ± 2.7 | 53 ± 1.9 | 2 × 103 |
Xylose, pH = 7.5 | wt | 24 ± 1.5 | 60 ± 2 | 2.5 × 103 |
 | rec | 23 ± 0.7 | 61 ± 1 | 2.7 × 103 |