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Figure 4 | Microbial Cell Factories

Figure 4

From: Enhanced expression of membrane proteins in E. coli with a PBAD promoter mutant: synergies with chaperone pathway engineering strategies

Figure 4

Reduced transcription and TF-inactivation synergistically improve the yields of pSRII. (A) Wild type (tig+, closed symbols) and TF-deficient cells (Δtig, open symbols) harboring the indicated plasmids were grown in LB-kanamycin at 37°C and pSRII synthesis was induced by addition of 0.2% L-arabinose in mid-exponential phase (arrow). (B) Membrane fractions corresponding to identical numbers of cells were collected 3 h post-induction and analyzed for pSRII content by SDS-PAGE (top) or immunoblotting (bottom) with anti-His antibodies. The arrow shows the migration position of pSRII while numbers below the blot quantify the intensity of each band relative to an arbitrary value of 1 for control cells. (C) Influence of plasmid and genetic background on the total yield of pSRII 3 h post-induction.

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Microbial Cell Factories

ISSN: 1475-2859

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