Figure 2

Combining pHtdR400-based expression with the use of TF-deficient strains leads to large gain in HtdR productivity. (A) Membrane fractions corresponding to identical numbers of cells were collected 3 h post-induction and analyzed for HtdR content by SDS-PAGE (top) or immunoblotting (bottom) with anti-His antibodies. The arrow shows the migration position of HtdR while numbers below the blot quantify the intensity of each band relative to an arbitrary value of 1 for control cells. (B) Influence of plasmid and genetic background on the total yield of HtdR 3 h post-induction. (C) Absorption spectra of solubilized membranes isolated 3 h post-induction from the indicated cells.