Figure 4

Mining the impact of phosphate scarcity by using complementary gene expression data sets. A. Venn diagram representing the overlapping genes identified with the DEA approach (comparison of cultures growing at low and high phosphate levels) and the SPA approach (consecutive analysis of gene expression patterns as a function of phosphate supply; gene numbers with increasing expression at low phosphate (dark red) and gene with decreasing expression at low phosphate (green) are highlighted. B. RT-PCR analysis of the transcript levels of insulin gene (blue) and the TPI1 gene (red) as a function of supplied phosphate; increased levels of the IAP transcript were measured under low phosphate levels; the mRNA levels are normalized to their initial quantities at high phosphate levels [33 mM]; cultures grown under limited phosphate supply [2.6 mM] showed decreased IAP and TPI1 transcript levels.