Figure 2
A volcano plot representation of the differentially expressed genes in a pair-wise comparison of cultures growing at high phosphate level [22 mM] to low phosphate level [9.6 mM]. The significance cut-off was set to a FDR of 0.05 (-log(adj.P.val ≥ 1.3), the biological cut-off was set to a fold change of ± 2 fold (-1 ≥ logFC ≥ 1), the top 30 differentially expressed genes are labeled with their corresponding gene ID, the absolute fold change of all genes are represented by the circle size, the five different color codes used represent insignificant genes (grey), both biologically and statistically significant genes being up-regulated (red, B) and down-regulated (green, A) at [22 mM] Pi, statistically but not biologically significant genes being up-regulated (orange) and down-regulated (light green) at [22 mM] Pi, respectively. A GO slim enrichment analysis of the gene sets A and B revealed the over-representation of the pictured GO terms within the gene sets A and B (%), the group of genes with an unknown biological function for gene set A (26%) and gene set B (25%) was excluded from further analysis.