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Table 5 Oligonucleotide sequences of the primers used in this study.

From: A thermostable GH45 endoglucanase from yeast: impact of its atypical multimodularity on activity

Orientation and primer

Sequence (5'-3')

Forward

 

   GH45CBMPiEcoF

GAATTC GCTCAAGCTGAAACTGCATCCC

   GH45PiEcoF

GAATTC GGAGACTTTGAGACAATCCCC

   GH45CBM1EcoF

GAATTC GATGTCCCACTTTGGGGCCAA

Reverse

 

   CBMPiXbaR

TCTAGA CCTGATGAAGTCGTTTCCTC

   GH45CBMPiXbaR

TCTAGA CCTTCGTCCGTACGAGCACA

  1. The restriction sites added to insert genes into pPICZαA are underlined.