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Table 4 inding parameters of Pp Cel45A and derived constructs on crystalline cellulose

From: A thermostable GH45 endoglucanase from yeast: impact of its atypical multimodularity on activity

Enzyme components

K d (μM)

Bmax (μmol bound per g of substrate)

Pp Cel45A

0.45

0.199

Pp CBM15:GH

0.95

0.794

Pp GH

n.d.

n.d.

Pp CBM11-5

0.78

0.185

  1. Binding parameters were determined using Avicel in duplicate and the K d and Bmax values were calculated using double-reciprocal plots. n.d. no binding detected.
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Microbial Cell Factories

ISSN: 1475-2859

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