Skip to main content

Table 1 Biochemical characterization of Pp Cel45A derived constructs

From: A thermostable GH45 endoglucanase from yeast: impact of its atypical multimodularity on activity

 

Purification yield

(mg/l) a

Molecular mass

(kDa)

Theoretical Experimental b

Apparent optimum

pH

Apparent optimum temperature (°C) c

Pp Cel45A

172

61.9

150

4.8

37-75 (65)

Pp CBM15:GH

250

31.5

80

4.8

37-75 (65)

Pp GH

215

22.6

35

4.8

37-75 (65)

Pp CBM11-5

68

35.2

134

n.d.

n.d.

  1. a expression yield is expressed in mg of recombinant protein purified per litre of culture
  2. b values are estimated from SDS-PAGE
  3. c values indicate that relative enzyme activity remaining was above 50%. Values in brackets indicate apparent optimum temperature under the conditions used
  4. n.d. : not determined since Pp CBM11-5 displays no catalytic activity.