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Table 3 Microfiltration experiment results

From: High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

  MF A MF B MF C
Time (h) 42 42 46
Maximal biomass concentration (gcdw·L-1) 23.90 31.70 36.40
μFB phase (h-1) 0.21 0.21 0.21
μMF phase (h-1) 0.10 0.13 0.12
Overall Y X/Glycerol (gcdw·g-1) 0.186 0.197 0.204
Culture supernatant K4 CPS concentration (gK4CPS·L-1) 2.03 3.00 4.33
Total K4 CPS produced (gK4CPS·L-1) 2.73 3.43 4.73
Overall Y K4 CPS/Glycerol (gK4CPS·g-1) 0.021 0.022 0.027
Y K4 CPS/X (gK4CPS·gcdw-1) 0.067 0.108 0.130
Productivity K4 CPS (gK4CPS·L-1·h-1) 0.065 0.082 0.103
J MF 12-24 h [L·(m2·h)-1] 1.71 1.62 1.63
J MF 25h-end [L·(m2·h)-1] 0.88 1.1 0.91
Permeate total volume (L) 5.60 6.50 6.10
Exchanged volumes (L·L-1) 3.10 3.60 3.40
  1. Parameters and data obtained from the E. coli K4 three different MF experiments. All the standard deviations were less than 5%.