High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

Table 3 Microfiltration experiment results

	MF A	MF B	MF C
Time (h)	42	42	46
Maximal biomass concentration (g_cdw·L^-1)	23.90	31.70	36.40
μFB phase (h^-1)	0.21	0.21	0.21
μMF phase (h^-1)	0.10	0.13	0.12
Overall Y_X/Glycerol (g_cdw·g^-1)	0.186	0.197	0.204
Culture supernatant K4 CPS concentration (g_K4CPS·L^-1)	2.03	3.00	4.33
Total K4 CPS produced (g_K4CPS·L^-1)	2.73	3.43	4.73
Overall Y_{K4 CPS/Glycerol} (g_K4CPS·g^-1)	0.021	0.022	0.027
Y_{K4 CPS/X} (g_K4CPS·g_cdw^-1)	0.067	0.108	0.130
Productivity K4 CPS (g_K4CPS·L^-1·h^-1)	0.065	0.082	0.103
J_{MF 12-24 h} [L·(m²·h)^-1]	1.71	1.62	1.63
J_{MF 25h-end} [L·(m²·h)^-1]	0.88	1.1	0.91
Permeate total volume (L)	5.60	6.50	6.10
Exchanged volumes (L·L^-1)	3.10	3.60	3.40

Parameters and data obtained from the E. coli K4 three different MF experiments. All the standard deviations were less than 5%.

ISSN: 1475-2859