Skip to main content

Table 3 Experimental design for starch and ammonium optimisation

From: Optimisation of batch culture conditions for cyclodextrin glucanotransferase production from Bacillus circulans DF 9R

Flask n°

Starch (y) g/L

Ammonium Sulphate (x) g/L

CGTase Activity

   

Rep1*

Rep2*

   

(U/mL)

1

20

4

3.36

3.37

2

35

4

1.22

0.93

3

5

4

1.30

1.65

4

10

6

2.25

2.48

5

10

2

2.77

3.19

6

30

6

1.09

1.58

7

30

2

1.54

1.74

8

20

7

3.30

3.10

9

20

1

1.82

2.05

  1. Other components of the culture medium were 0.02 g/L MgSO4.7H2O, 1.78 g/L KH2PO4, 15.14 g/L K2HPO4, 0.02 g/L FeSO4 (pH 8.0) at 37°C. * Replicate experiments
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us