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Table 2 Effect of cation addition on growth and enzyme production

From: Optimisation of batch culture conditions for cyclodextrin glucanotransferase production from Bacillus circulans DF 9R

Cation added*

Growth (OD620 nm)

CGTase activity (U/mL)

none

0.15 ± 0.1

0

Mg2+

1.10 ± 0.1

0

Ca2+

1.30 ± 0.05

0.2 ± 0.05

Fe2+

1.66 ± 0.1

1.5 ± 0.1

Mn2+, Co2+, Ni2+ or Zn2+

1.30 ± 0.1

0.1 ± 0.05

  1. * Basal medium was prepared in deionized water and supplemented with 0.2 g/L of the corresponding mineral salt. For testing the effect of Ca2+, Fe2+, Mn2+, Co2+, Ni2+ and Zn2+ the basal medium was supplemented with Mg2+.
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Microbial Cell Factories

ISSN: 1475-2859

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