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Table 1 Effect of carbon and nitrogen sources on growth and enzyme production

From: Optimisation of batch culture conditions for cyclodextrin glucanotransferase production from Bacillus circulans DF 9R

Nutrient

Growth (OD620nm)

CGTase activity (U/mL)

Carbon source (10 g/L)

  

Potato soluble starch

1.64 ± 0.18

2.4 ± 0.06

Corn starch

1.65 ± 0.04

2.2 ± 0.08

Rice starch

1.24 ± 0.02

2.8 ± 0.06

Wheat starch

1.37 ± 0.03

2.0 ± 0.07

Cassava starch

2.07 ± 0.04

2.9 ± 0.04

Nitrogen source (4 g/L)

  

Ammonium chloride

1.26 ± 0.02

3.60 ± 0.1

Ammonium nitrate

1.03 ± 0.01

3.55 ± 0.05

Ammonium sulfate

1.30 ± 0.1

3.55 ± 0.1

Ammonium plus 0.5% tryptone

2.55 ± 0.1

1.50 ± 0.2

Ammonium plus 0.5% corn steep liquor

2.75 ± 0.2

1.15 ± 0.1

  1. Basal medium containing 0.02 g/L MgSO4.7H2O, 1.78 g/L KH2PO4, 15.14 g/L K2HPO4, 0.02 g/L FeSO4 (pH 7.6) at 37°C with 2 g/L (NH4)2SO4 as nitrogen source was used when carbon sources were tested, and 10 g/L cassava starch when nitrogen sources tested. Microbial growth (and enzyme activity) was not detected in the presence of glucose, xylose, maltose, α or β-CDs as carbon sources.
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Microbial Cell Factories

ISSN: 1475-2859

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