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  1. Research

    SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

    The yeast Saccharomyces cerevisiae is increasingly used as a cell factory. However, cell factory construction time is a major obstacle towards using yeast for bio-production. Hence, tools to speed up cell factory...

    Katherina García Vanegas, Beata Joanna Lehka and Uffe Hasbro Mortensen

    Microbial Cell Factories 2017 16:25

    Published on: 8 February 2017

  2. Research

    Understanding the interplay of carbon and nitrogen supply for ectoines production and metabolic overflow in high density cultures of Chromohalobacter salexigens

    The halophilic bacterium Chromohalobacter salexigens has been proposed as promising cell factory for the production of the compatible solutes ectoine and hydroxyectoine. This bacterium has evolved metabolic adapt...

    María J. Salar-García, Vicente Bernal, José M. Pastor, Manuel Salvador, Montserrat Argandoña, Joaquín J. Nieto, Carmen Vargas and Manuel Cánovas

    Microbial Cell Factories 2017 16:23

    Published on: 8 February 2017

  3. Research

    Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica

    Droplet-based microfluidics is becoming an increasingly attractive alternative to microtiter plate techniques for enzymatic high-throughput screening (HTS), especially for exploring large diversities with lowe...

    Thomas Beneyton, Stéphane Thomas, Andrew D. Griffiths, Jean-Marc Nicaud, Antoine Drevelle and Tristan Rossignol

    Microbial Cell Factories 2017 16:18

    Published on: 31 January 2017

  4. Research

    Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

    Through functional screening of a fosmid library, generated from a phytopathogen-suppressive soil metagenome, the novel antifungal chitinase—named Chi18H8 and belonging to family 18 glycosyl hydrolases—was pre...

    Francesca Berini, Ilaria Presti, Fabrizio Beltrametti, Marco Pedroli, Kjell M. Vårum, Loredano Pollegioni, Sara Sjöling and Flavia Marinelli

    Microbial Cell Factories 2017 16:16

    Published on: 31 January 2017

  5. Research

    Dynamic control of ERG20 expression combined with minimized endogenous downstream metabolism contributes to the improvement of geraniol production in Saccharomyces cerevisiae

    Microbial production of monoterpenes provides a promising substitute for traditional chemical-based methods, but their production is lagging compared with sesquiterpenes. Geraniol, a valuable monoterpene alcoh...

    Jianzhi Zhao, Chen Li, Yan Zhang, Yu Shen, Jin Hou and Xiaoming Bao

    Microbial Cell Factories 2017 16:17

    Published on: 31 January 2017

  6. Review

    Microbial production of vitamin B12: a review and future perspectives

    Vitamin B12 is an essential vitamin that is widely used in medical and food industries. Vitamin B12 biosynthesis is confined to few bacteria and archaea, and as such its production relies on microbial fermentatio...

    Huan Fang, Jie Kang and Dawei Zhang

    Microbial Cell Factories 2017 16:15

    Published on: 30 January 2017

  7. Research

    Joining the in vitro immunization of alpaca lymphocytes and phage display: rapid and cost effective pipeline for sdAb synthesis

    Camelids possess unique functional heavy chain antibodies, which can be produced and modified in vitro as a single domain antibody (sdAb or nanobody) with full antigen binding ability. Production of sdAb in co...

    Lubos Comor, Saskia Dolinska, Katarina Bhide, Lucia Pulzova, Irene Jiménez-Munguía, Elena Bencurova, Zuzana Flachbartova, Lenka Potocnakova, Evelina Kanova and Mangesh Bhide

    Microbial Cell Factories 2017 16:13

    Published on: 23 January 2017

  8. Research

    Human β-defensin-2 production from S. cerevisiae using the repressible MET17 promoter

    Baker’s yeast Saccharomyces cerevisiae is a proven host for the commercial production of recombinant biopharmaceutical proteins. For the manufacture of heterologous proteins with activities deleterious to the hos...

    Thea S. B. Møller, Joanna Hay, Malcolm J. Saxton, Karen Bunting, Evamaria I. Petersen, Søren Kjærulff and Christopher J. A. Finnis

    Microbial Cell Factories 2017 16:11

    Published on: 18 January 2017

  9. Research

    Engineered biosynthesis of milbemycins in the avermectin high-producing strain Streptomyces avermitilis

    Milbemycins, produced from Streptomyces hygroscopicus subsp. aureolacrimosus and Streptomyces bingchenggensis, are 16-membered macrolides that share structural similarity with avermectin produced from Streptomyce...

    Myoun-Su Kim, Wan-Je Cho, Myoung Chong Song, Seong-Whan Park, Kaeun Kim, Eunji Kim, Naryeong Lee, Sang-Jip Nam, Ki-Hoon Oh and Yeo Joon Yoon

    Microbial Cell Factories 2017 16:9

    Published on: 17 January 2017

  10. Research

    Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

    Animal venoms are large, complex libraries of bioactive, disulphide-rich peptides. These peptides, and their novel biological activities, are of increasing pharmacological and therapeutic importance. However, ...

    Ana Filipa Sequeira, Jeremy Turchetto, Natalie J. Saez, Fanny Peysson, Laurie Ramond, Yoan Duhoo, Marilyne Blémont, Vânia O. Fernandes, Luís T. Gama, Luís M. A. Ferreira, Catarina I. P. I. Guerreiro, Nicolas Gilles, Hervé Darbon, Carlos M. G. A. Fontes and Renaud Vincentelli

    Microbial Cell Factories 2017 16:4

    Published on: 17 January 2017

  11. Research

    CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

    Anthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins ...

    Brady F. Cress, Quentin D. Leitz, Daniel C. Kim, Teresita D. Amore, Jon Y. Suzuki, Robert J. Linhardt and Mattheos A. G. Koffas

    Microbial Cell Factories 2017 16:10

    Published on: 17 January 2017

  12. Research

    High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery

    Animal venoms are complex molecular cocktails containing a wide range of biologically active disulphide-reticulated peptides that target, with high selectivity and efficacy, a variety of membrane receptors. Di...

    Jeremy Turchetto, Ana Filipa Sequeira, Laurie Ramond, Fanny Peysson, Joana L. A. Brás, Natalie J. Saez, Yoan Duhoo, Marilyne Blémont, Catarina I. P. D. Guerreiro, Loic Quinton, Edwin De Pauw, Nicolas Gilles, Hervé Darbon, Carlos M. G. A. Fontes and Renaud Vincentelli

    Microbial Cell Factories 2017 16:6

    Published on: 17 January 2017

  13. Research

    Improvement of yeast tolerance to acetic acid through Haa1 transcription factor engineering: towards the underlying mechanisms

    Besides being a major regulator of the response to acetic acid in Saccharomyces cerevisiae, the transcription factor Haa1 is an important determinant of the tolerance to this acid. The engineering of Haa1 either ...

    Steve Swinnen, Sílvia F. Henriques, Ranjan Shrestha, Ping-Wei Ho, Isabel Sá-Correia and Elke Nevoigt

    Microbial Cell Factories 2017 16:7

    Published on: 9 January 2017

  14. Research

    The ACEII recombinant Trichoderma reesei QM9414 strains with enhanced xylanase production and its applications in production of xylitol from tree barks

    ACEII transcription factor plays a significant role in regulating the expression of cellulase and hemicellulase encoding genes. Apart from ACEII, transcription factors such as XYR1, CRE1, HAP2/3/5 complex and ...

    Lili Xiong, Ayyappa Kumar Sista Kameshwar, Xi Chen, Zhiyun Guo, Canquan Mao, Sanfeng Chen and Wensheng Qin

    Microbial Cell Factories 2016 15:215

    Published on: 28 December 2016

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