Volume 5 Supplement 1
Addition of Repressor in inducible promoter system improves soluble expression of recombinant protein in E. coli
- Kyung-Hwan Jung1
© Jung; licensee BioMed Central Ltd. 2006
Published: 10 October 2006
Insoluble protein aggregate (inclusion body) is frequently accumulated during the heterologous protein expression by the bacterial inducible promoter system. In this study, although many reports have proposed the methodology to circumvent the aggregate formation [1–3], we tried to control the transcription rate by an addition of the repressor for inducible promoter. The addition of repressor was tried just after the inducer was added, in order to increase the soluble expression level.
The glucose (repressor) addition improved the soluble expression level in arabinose-inducible promoter system in E. coli.
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This article is published under license to BioMed Central Ltd.