Volume 5 Supplement 1

The 4th Recombinant Protein Production Meeting: a comparative view on host physiology

Open Access

Comparative transcriptional profiling of the bacterial stress response in temperature and chemically-induced recombinant E. coli processes

  • Daniela Böhm1 and
  • Ursula Rinas1
Microbial Cell Factories20065(Suppl 1):P1

DOI: 10.1186/1475-2859-5-S1-P1

Published: 10 October 2006

Background

Production of heterologous proteins results in a number of metabolic and physiological changes in the host cells during the course of a production process, namely the induction of stress responses and corresponding alterations in gene expression profiles [1].

Results

This study focuses on quantitative monitoring of the adaptation of E. coli to recombinant protein production on the transcriptome level by a bead-based RNA sandwich hybridisation assay, a rapid novel method based on the detection of hybridisation events between specific oligonucleotide probes and the target nucleic acids [2, 3].

The expression profiles of selected genes including the product gene, anabolic and stress responsive genes were quantitatively analyzed in cells producing the human basic fibroblast growth factor (hFGF-2), a protein that partially aggregates into inclusion bodies. Transcriptome profiles during temperature- and IPTG-induced synthesis of hFGF-2 using the K12 strain TG1 and BL21(DE3) as production hosts, respectively, were compared.

Authors’ Affiliations

(1)
GBF – German Research Centre for Biotechnology

References

  1. Hoffmann F, Rinas U: Stress induced by recombinant protein production in Escherichia coli. Adv Biochem Eng Biotechnol. 2004, 89: 73-92.Google Scholar
  2. Gabig-Ciminska M, Holmgren A, Andresen H, Barken K, Wümpelmann M, Albers J, Hintsche R, Breitenstein A, Neubauer P, Los M, et al.: Electric chips for rapid detection and quantification of nucleic acids. Biosens Bioelectron. 2004, 19: 537-546. 10.1016/S0956-5663(03)00273-2.View ArticleGoogle Scholar
  3. Soini J, Falschlehner C, Mayer C, Böhm D, Panula J, Vasala A, Neubauer P: Transient increase of ATP as a response to temperature up-shift in Escherichia coli. Microb Cell Fact. 2005, 4: 9- 10.1186/1475-2859-4-9.View ArticleGoogle Scholar

Copyright

© Böhm and Rinas; licensee BioMed Central Ltd. 2006

This article is published under license to BioMed Central Ltd.

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