Metabolic engineering of microbes for oligosaccharide and polysaccharide synthesis
© Ruffing and Chen; licensee BioMed Central Ltd. 2006
Received: 26 December 2005
Accepted: 21 July 2006
Published: 21 July 2006
Metabolic engineering has recently been embraced as an effective tool for developing whole-cell biocatalysts for oligosaccharide and polysaccharide synthesis. Microbial catalysts now provide a practical means to derive many valuable oligosaccharides, previously inaccessible through other methods, in sufficient quantities to support research and clinical applications. The synthesis process based upon these microbes is scalable as it avoids expensive starting materials. Most impressive is the high product concentrations (up to 188 g/L) achieved through microbe-catalyzed synthesis. The overall cost for selected molecules has been brought to a reasonable range (estimated $ 30–50/g).
Microbial synthesis of oligosaccharides and polysaccharides is a carbon-intensive and energy-intensive process, presenting some unique challenges in metabolic engineering. Unlike nicotinamide cofactors, the required sugar nucleotides are products of multiple interacting pathways, adding significant complexity to the metabolic engineering effort. Besides the challenge of providing the necessary mammalian-originated glycosyltransferases in active form, an adequate uptake of sugar acceptors can be an issue when another sugar is necessary as a carbon and energy source. These challenges are analyzed, and various strategies used to overcome these difficulties are reviewed in this article. Despite the impressive success of the microbial coupling strategy, there is a need to develop a single strain that can achieve at least the same efficiency. Host selection and the manner with which the synthesis interacts with the central metabolism are two important factors in the design of microbial catalysts. Additionally, unlike in vitro enzymatic synthesis, product degradation and byproduct formation are challenges of whole-cell systems that require additional engineering. A systematic approach that accounts for various and often conflicting requirements of the synthesis holds the key to deriving an efficient catalyst.
Metabolic engineering strategies applied to selected polysaccharides (hyaluronan, alginate, and exopolysaccharides for food use) are reviewed in this article to highlight the recent progress in this area and similarity to challenges in oligosaccharide synthesis. Many naturally occurring microbes possess highly efficient mechanisms for polysaccharide synthesis. These mechanisms could potentially be engineered into a microbe for oligosaccharide and polysaccharide synthesis with enhanced efficiency.
Oligosaccharides and polysaccharides are found in nature as components of a broad range of molecular structures, such as cell surface glycoproteins and glycolipids. Carbohydrate moieties of these structures play vital roles in cellular communication processes, as points of attachment for antibodies and other proteins, and as receptors for bacteria and viral particles . Oligosaccharides and polysaccharides therefore have enormous potential as therapeutic agents. However, this potential is far from being realized due to the complex structure of oligosaccharides which makes classical chemical synthesis difficult . Biocatalytic strategies, using either enzymes or whole-cells, represent a realistic and scalable approach to the enormous challenges in synthesizing complex carbohydrates. In this review, we focus on advances made in recent years in developing efficient microbial whole-cell catalysts through metabolic engineering. Due to space limitations, enzymatic approaches, where isolated enzymes are used, are not covered here. Several recent reviews provide excellent overviews of research activities utilizing the enzymatic approach [3–7].
Oligosaccharide structures synthesized with metabolically engineered microbes
Metabolic engineering of microbial cells represents one of the most promising strategies for deriving oligosaccharides in sufficient quantities to support research and clinical development. Structures synthesized using metabolically engineered microbes reported in literature since 1998 were surveyed and are tabulated in Table 1 (see Additional file 1). Oligosaccharides, di-, tri-, tetra-, and penta-saccharides of diverse structures were successfully synthesized with engineered Escherichia coli, Pichia pastoris, Agrobacterium sp. Corneybacterium ammoniagenes, and Corneybacterium glutamicum. The sugar nucleotide regeneration is central to these metabolic engineering efforts, and many ingenious methods were developed. Depending on the specific sugar nucleotide, the methods vary considerably in the degree of complication. With only one exception (truncated bovine α-1,3-galactosyltransferase), these syntheses exploited microbial glycosyltransferase enzymes, many of which were originated from microbial pathogens. One important advantage of the whole-cell approach is the scalability. Since it avoids expensive starting materials and enzyme isolation, once the strain is developed, it can be easily scaled-up in a fermentor to produce the needed quantity of compounds. This is reflected in the scale of synthesis that has been carried out (up to 2 L, Table 1; see Additional file 1). Product concentration was also impressive, especially in the microbial coupling approach. A concentration as high as 188 g/L (372 mM), highest ever reported for oligosaccharides, was obtained .
The following sections detail various metabolic engineering strategies used in providing the necessary elements for synthesis (donors, acceptors, glycosyltransferases). In addition, several unique metabolic engineering challenges in oligosaccharide synthesis are analyzed.
Donor (or sugar nucleotide) provision in oligosaccharide synthesis is very challenging. The sheer number of enzymes involved in the synthesis of a sugar nucleotide makes an enzymatic approach less desirable for large-scale synthesis. Consequently, whole-cell catalysts were adopted for their virtue of facile cofactor provisions.
Since UDP-glucose is a precursor in cell wall biosynthesis, all bacteria are capable of its synthesis, at least at a basal level. Although UDP-glucose has not directly been used in oligosaccharide synthesis, its efficient regeneration is important as a precursor for synthesis of other sugar nucleotides such as UDP-galactose and UDP-GA.
Like UDP-glucose, UDP-GlcNAc is a precursor to cell wall synthesis. As such, the endogenous UDP-GlcNAc pool can be directly used for oligosaccharide synthesis, and its native synthesis pathway can be used for its regeneration .
The only report on UDP-GalNAc as a donor in oligosaccharide synthesis was from Samain and coworkers . In this method, the endogenous UDP-GlcNAc was epimerized to UDP-GalNAc using an UDP-GlcNAc 4'-epimerase (WbpP) derived from Pseudomonas aeruginosa O6.
Regeneration systems for GDP-Man and UDP-GA have so far not been described in the literature for oligosaccharide synthesis. However, strategies developed for GDP-fucose could also be applied to GDP-Man, a precursor to GDP-fucose. Similarly, a strategy could be devised for regeneration of UDP-GA by introducing UDP-glucose dehydrogenase into the UDP-glucose synthesis pathway.
A unique problem in oligosaccharide synthesis with metabolically engineered cells is the entry of acceptor sugars. The energy requirement of the synthesis (except in the case of sucrose synthase) necessitates a supply of energy source, most often glucose. Due to selective uptake of this energy source, uptake of the acceptor is not efficient. Several strategies have been developed to improve acceptor uptake. Permeabilization by chemical treatment or freeze-thaw was generally effective in bringing the acceptor sugars (such as lactose and GlcNAc) inside the cells; however, it dissipates the membrane potential required for energy metabolism. Acceptor sugars could be generated inside the cell by introducing suitable enzymes. For example, Samain et al. developed a method to generate chitin oligosaccharide (acceptor sugar) in-situ, which was then further modified to yield chitobiose  or was further elaborated through introduction of LgtB to generate a LacNAc moiety . Another method is through the manipulation of the catabolite operon of the acceptor sugars. Significant intracellular accumulation of lactose was achieved by knocking out the lacZ gene (encoding β-galactosidase) while leaving lacY (lactose permease) intact . An intracellular lactose accumulation of 7–8 mM was observed when the extracellular concentration was 14.6 mM (5 g/L). As a result, lactose could serve as the acceptor for in vivo reactions. Several valuable tri-, tetra-, and penta-saccharides were successfully synthesized using this method [13, 19].
In in vitro enzymatic synthesis, where the supply of donor and acceptor sugars can be conveniently controlled, unwanted byproduct formation is not generally a problem. However, when the synthesis is taking place inside the cell, many byproducts could arise due to a number of reasons. Many glycosyltransferases are promiscuous. LgtB from Neisseria meningitidis, for example, can use GlcNAc and glucose, among others, as acceptors, leading to synthesis of LacNAc and lactose, respectively . Although GlcNAc is a slightly favored acceptor as compared to glucose, under in vivo conditions, the presence of glucose could lead to a condition that greatly favors the synthesis of lactose. Lactose formation is detrimental to the synthesis process not only causing purification issues, but also draining the UDP-galactose and UTP pools , which could otherwise be channeled into LacNAc synthesis.
Interaction of oligosaccharide synthesis with central metabolism
With the exception of using sucrose synthase for UDP-glucose (and UDP-Gal) regeneration, where the co-product UDP can directly activate the sugar to generate UDP-glucose, oligosaccharide synthesis is intimately dependent upon cellular metabolism. In the case of sucrose synthase, the host is not an active player in the synthesis, it merely provides a milieu in which enzymatic reactions take place. Carbon metabolism is not needed to provide precursors or energy source for the oligosaccharide synthesis. This contrasts with the usual scenario, where a high energy compound such as UTP, GTP, CTP, ATP, or equivalent such as PEP is needed for activation of sugars in each cycle of the reaction. This necessarily brings in the complexity of central metabolism to the oligosaccharide synthesis. Consequently, an energy source is needed, and the carbon flux distributions need to be considered. Since the best synthesis has been demonstrated with bacterial coupling, it is instructive to analyze the energy source and carbon flux for this case. High-energy compounds are produced from orotic acid by a strain efficient in nucleotide synthesis (Figures 2, 5, and 7). The UTP or other equivalents are utilized by the coupled E. coli strain(s) engineered to overproduce sugar nucleotides, which in turn are consumed by a strain harboring the necessary glycosyltransferases. This strategy avoids the complication of coupling central metabolism for energy production with the oligosaccharide synthesis by using multiple types of cells, each with a specialized role. Although so far this strategy gives the highest product concentration and provides a realistic technology for commercialization, the requirement of at least three fermentations for a single glycosidic bond makes it a far from ideal solution to a complicated problem. So logically, one asks whether it is possible to achieve the same efficiency in a single strain. From a material balance point of view, the synthesis of an oligosaccharide does not require a constant supply of a nitrogen source, such as orotic acid, if the co-product UDP is truly recycled. It should only require a carbon and energy source to support the synthesis. A single strain strategy could work as efficiently as three, four, or five coupled strains. This assertion comes from the observation of microbial polysaccharide synthesis. Polysaccharide synthesis shares much of the same challenges as oligosaccharide synthesis, being both carbon and energy intensive. Yet successful microbes are found to efficiently channel the carbons to polysaccharides with comparable product yield, product concentration, and productivity to what was reported with the microbial coupling strategy. Examples of those are curdlan-producing Agrobacterium sp. and xanthan gum-producing Xanthomonas campestris. Therefore, the key to success for a single strain strategy seems to rely on uncovering the secret of polysaccharide synthesis from these microbes, particularly with regard to understanding the coupling of central metabolism to polysaccharide synthesis. The available genome sequences along with proteomic and metablomic tools should facilitate the study toward this goal.
This section only concerns the single strain strategy. A successful oligosaccharide synthesis system, as it becomes clear from the above analysis, requires active glycosyltransferases, an efficient in-situ provision mechanism of donors, internalization of acceptors, and a continuous supply of energy. A host must be able to express the requisite glycosyltransferase enzymes in active forms. Despite impressive successful examples, this is not always the case, especially considering that many interesting glycans are of mammalian-origin. Bacterial glycosyltransferases are extremely useful to avoid the difficulty in expression, but one cannot always find a bacterial version of the glycosyltransferase enzyme required for the synthesis. Therefore, expression of active glycosyltransferases is an important consideration in the choice of a host strain for the metabolic engineering effort. Only a limited number of hosts were used for oligosaccharide synthesis, mainly those that have established genetic tools and commercially available expression vectors.
Donor provision is a tough issue in the engineering process. As reviewed in earlier sections, a strategy to use the cells' natural mechanism for donor regeneration has been successfully used without much genetic manipulation. On the other hand, strategies to create new regeneration mechanisms were also developed. Another interesting strategy has emerged which uses a host that naturally overproduces a sugar polymer. By appropriate engineering, the already efficient mechanism for cofactor regeneration can be harnessed for oligosaccharides synthesis. This strategy was illustrated by Rigngenberg et al. in an E. coli strain producing polysialic acid . A null defect in the gene encoding polysialyltransferase (neuS) dramatically altered the sialate metabolism, allowing accumulation of CMP-sialic acid to a level more than 50 times higher than that of the wild type. Although this was not demonstrated for sialyl-oligosaccharide synthesis, it is conceivable that by expressing a sialyltransferase and providing a suitable acceptor, the strain could be used for sialyl-oligosaccharide synthesis. In the authors' lab, an Agrobacterium sp. strain, naturally overproducing curdlan (a β1,3-glucose homopolymer), was engineered for UDP-galactose regeneration by reducing glucose polymerization. Although the UDP-glucose pool was not significantly elevated by the mutation, oligosaccharide synthesis was much higher than that in an E. coli strain under comparable conditions . These examples show that judicious selection of a host strain can lead to an efficient system without overexpression of the enzymes involved in cofactor regeneration. The use of naturally evolved strains for sugar nucleotide regeneration is superior in not only minimizing the genetic modification and resulting metabolic burden of recombinant protein expression, but also ensuring optimal pathway interaction.
Overall, the in-situ provision of donors, acceptor access, and interaction of central metabolism for oligosaccharide synthesis require significant host modification beyond high expression of certain enzymes; therefore, the amenability of the host to genetic engineering is an important consideration in the choice of a host. Broad-host-range expression vectors can be developed for gram-negative bacteria, which allow the introduction of novel enzymes into a wide range of strains, widening the selection of host strains.
The following sections highlight recent metabolic engineering efforts in improving production of sugar polymers. Space constraints make it necessary for us to limit the review to only a few polymers.
Hyaluronic acid (HA) or hyaluronan is a linear polymer of a repeating disaccharide unit, glucuronic acid (GlcA) and N-acetyl-glucosamine (GlcNAc). It is found in all vertebrates as a major constituent of the extracellular matrices. In animals, it is found in the vitreous body of the eye, the synovial fluid of articular joints, and the intercellular space of the epidermis. HA is believed to be a lubricant between joint surfaces. It is also involved in morphogenesis and differentiation. Additionally, HA interacts with proteins such as CD44, RHAMM, and fibrinogen, influencing many biological processes such as angiogenesis, cancer, cell motility, wound healing, and cell adhesion . Due to these varied roles, HA influences cell behavior and has significant structural, rheological, physiological, and biological functions in the body. Its distinctive viscoelastic properties, coupled with its lack of immunogenicity and toxicity, have led to a wide range of applications in the cosmetic and medicinal fields, including skin moisturizers, osteoarthritis treatment, ophthalmic surgery, adhesion prevention after abdominal surgery, and wound healing . HA can be either extracted from rooster combs or obtained through fermentation with strains of group C Streptococcus. In rooster combs, HA is complexed with proteoglycans, making the isolation of high purity, high molecular weight HA costly . In addition, animal-derived therapeutics carry the risk of transmitting viruses and other adventitious agents as well as inducing immunogenic and inflammatory responses. Due to difficulties in genetic engineering, the Streptococcus fermentation route is far from ideal despite its long history as a production strain of HA.
HA is synthesized from two precursors, UDP-glucuronic Acid (UDP-GA) and UDP-N-acetylglucosamine (UDP-GlcNAc), by a polymerase called hyaluronan synthase (encoded by has A). UDP-GA can be obtained from oxidation of UDP-glucose, which is catalyzed by the NADH-dependent UDP-glucose dehydrogenase. Both UDP-glucose and UDP-GlcNAc are precursors for cell wall biosynthesis. HA synthesis therefore competes with cell growth. Recognizing the high energy demand of HA synthesis, metabolic engineering of Streptococcus to improve energy production was attempted by overexpressing NADH oxidase (NOX) and thereby shifting the metabolism from lactate-producing to acetate-producing (energy making) mixed fermentation. This strategy successfully increased the ATP yield by 30%. However, it only led to a 15% biomass increase instead of the desired increase in HA synthesis . This illustrates the difficulty of improving production of a product whose synthesis competes with biomass synthesis in precursor and energy requirements. A successful metabolic engineering strategy will necessarily delineate biomass production from HA synthesis. Other metabolic engineering efforts have been focused on developing suitable microbial production strains. Recently, Novozyme has developed a production system with a Bacillus subtilis strain . Although there are no benefits in productivity and HA molecular weight as compared to the Streptococcus fermentation, Bacillus is a better production strain as it is a GRAS (generally regarded as safe) and the HA produced is not cell-associated, facilitating downstream processing . Another HA synthesis system was developed using chlorella cells infected with Chlorovirus . The yield is about 10 times lower than that of other microbial systems. However, a potential advantage of the system is the higher molecular weight product. This is important as many HA applications are molecular weight dependent. In fact, higher molecular weight HA is highly prized in medical applications such as eye surgery. Derivation of a production strain capable of making high molecular weight HA with mono-dispersity is a holy grail for metabolic engineering efforts. The success of such endeavors awaits a full understanding of the chain termination mechanisms in HA polymer synthesis.
Alginate is a linear copolymer of two-sugar residues, β-D-mannuronic acid (M) and α-L-guluronic acid (G), linked together by 1–4 linkages . The industrially important polymer is produced commercially by extraction from brown seaweeds. Several microbes, such as Psudomonas aruginosa and Azotobacter vinelandii, naturally produce alginate, and a microbial production method could potentially be developed. As properties of the polymer vary widely with the monomer sugar sequence, microbial systems offer exciting opportunities to produce tailor-made polymers with desired properties. Key to the success of such endeavors is the understanding of enzyme functions involved in alginate synthesis. Of particular interest is the mannuronan C-5-epimerase, which catalyzes post-polymerization conversion of M residues to G residues. Although Psudomonas aruginosa encodes only one such enzyme, Azotobacter vinelandii has up to 8 epimerases that differ in how the epimerization is introduced, leading to either alternating or G blocks (stretches of consecutive G residues) of different lengths, and thereby considerable diversity of the structure and property. These enzymes have interesting composite structures, containing multiple copies of domains of different functions. This structural feature provides particularly good metabolic engineering targets for rational engineering of polymer properties, provided that the gene-to-enzyme activity relationships are understood in sufficient detail.
Exopolysaccharides for food use
Exopolysaccharides (EPS) made by bacteria such as Lactococcus and Streptococcus are an active subject of metabolic engineering. These organisms are widely used in dairy product production. In-situ exopolysaccharide synthesis allows modulation of rheology, improved mouthfeel, and textures of food products. It can also impart some health benefits as prebiotics. However, EPS represents significant metabolic engineering challenges as the natural production level is generally quite low, 50–400 mg/L, when compared to other food-grade polysaccharides such as xanthan (25 g/L, ) and curdlan (93 g/L, ) made by non-dairy organisms. Metabolic engineering efforts in improving EPS production have been directed to precursor synthesis. Overexpression of UDP-glucose pyrophosphorylase (GalU), an enzyme involved in UDP-glucose synthesis and under the control of a nisin inducible promoter, increased the enzyme specific activity in Lactococcus lactis by 20-fold, which in turn increased both UDP-glucose and UDP-galactose synthesis by eight-fold. However, the increased enzyme specific activities did not significantly enhance the synthesis of EPS . Similarly, overexpression of the phosphoglucomutase gene (pgm) in Sphingomonas, led to a six-fold increase in enzyme specific activity, yet only a small percentage of increase in exopolysaccharides . It appears that, at least in these two examples, the level of precursors was not limiting the EPS synthesis. However, overexpression of both PGM (the enzyme catalyzing the conversion of glucose-6-phosphate to glucose-1-phosphate) and GalU in Streptococcus thermophilus led to a two-fold increase in EPS synthesis . Further increase (50%) was obtained when Leloir enzyme (GalK, GalT, and GalU) activities were also increased. While precursor synthesis and interaction with central metabolism are the main focus, other metabolic engineering strategies were also pursued. The increase in the expression of biosynthetic gene cluster in Sphingomonas (including glycosyltransferase enzyme activity), for example, improved EPS synthesis by 20% . Further improvement in the production level, an order of magnitude improvement, is desirable for most applications. Therefore, significant challenges still lie ahead. Potentially, not only can the production level be increased, but the composition of EPS could be modified or tailored to target applications.
Tremendous progress has been made in recent years in complex carbohydrate synthesis. Metabolic engineering has provided the tools for development of highly efficient microbial whole-cell catalysts for oligosaccharide and polysaccharide synthesis. Product concentrations as high as 188 g/L were obtained with engineered strains. An important advantage of using metabolically engineered whole-cell catalyst versus other approaches is the ability to use inexpensive starting material. Consequently, the synthesis could be readily scaled-up according to need. Many valuable oligosaccharides have now been available in gram or larger quantities at realistic costs. An economic analysis puts the cost of oligosaccharide synthesis at $30–50/g . This indeed should be hailed as a significant achievement of metabolic engineering. However, significant challenges still lie ahead. Although the structures synthesized through the engineered strains listed in Table 1 (see Additional file 1) are very impressive, they are only a small fraction of nature's diversity. Many structures of medical importance still await an efficient method for synthesis. For those structures already successfully synthesized, it is important to achieve high efficiency of synthesis in a single strain, as opposed to using coupled microbes, to make the process economically viable.
cytidine monophosphate-N-acetylneuraminic acid
- CTP cytidine triphosphate; Fru-6-P:
- Man-1-P mannose-1-phosphate; Man-6-P:
nicotinamide adenine dinucleotide phosphate (oxidized)
nicotinamide adenine dinucleotide phosphate (reduced)
- PEP phosphoenolpyruvate; Pi:
uridine diphosphate-glucuronic acid
uridine diphosphate-N- acetylglucosamine
Work in the authors' lab is supported by National Science Foundation and the American Cancer Society. A. Ruffing acknowledges a NSF graduate fellowship.
- Wong C-H, Whitesides GM: Enzymes in Synthetic Organic Chemistry. New York: Oxford, 12: 1
- Perugino G, Trincone A, Rossi M, Moracci M: Oligosaccharide synthesis by glycosynthases. Trends in Biotechnology. 2004, 22 (1): 31-37. 10.1016/j.tibtech.2003.10.008.View ArticleGoogle Scholar
- Saxon E, Bertozzi CR: Chemical and biological strategies for engineering cell surface glycosylation. Annual Review of Cell and Developmental Biology. 2001, 17: 1-23. 10.1146/annurev.cellbio.17.1.1.View ArticleGoogle Scholar
- Flitsch SL: Chemical and enzymatic synthesis of glycopolymers. Current Opinion in Chemical Biology. 2000, 4 (6): 619-625. 10.1016/S1367-5931(00)00152-6.View ArticleGoogle Scholar
- Koeller KM, Wong CH: Complex carbohydrate synthesis tools for glycobiologists: enzyme-based approach and programmable one-pot strategies. Glycobiology. 2000, 10 (11): 1157-1169. 10.1093/glycob/10.11.1157.View ArticleGoogle Scholar
- Crout DH, Vic G: Glycosidases and glycosyl transferases in glycoside and oligosaccharide synthesis. Current Opinion in Chemical Biology. 1998, 2 (1): 98-111. 10.1016/S1367-5931(98)80041-0.View ArticleGoogle Scholar
- Meynial-Salles I, Combes D: In vitro glycosylation of proteins: An enzymatic approach. Journal of Biotechnology. 1996, 46 (1): 1-14. 10.1016/0168-1656(95)00174-3.View ArticleGoogle Scholar
- Koizumi S, Endo T, Tabata K, Ozaki A: Large-scale production of UDP-galactose and globotriose by coupling metabolically engineered bacteria. Nature Biotechnology. 1998, 16: 847-850. 10.1038/nbt0998-847.View ArticleGoogle Scholar
- Liu Z, Lu Y, Zhang J, Pardee K, Wang PG: P1 trisaccharide (Galα1,4Galβ1,4GlcNAc) synthesis by enzyme glycosylation reactions using recombinant Escherichia coli. Applied and Environmental Microbiology. 2003, 69 (4): 2110-2115. 10.1128/AEM.69.4.2110-2115.2003.View ArticleGoogle Scholar
- Zhang J, Kowal P, Chen X, Wang PG: Large-scale synthesis of globotriose derivatives through recombinant E. coli. Org Biomol Chem. 2003, 1: 3048-3053. 10.1039/b304911f.View ArticleGoogle Scholar
- Bettler E, Imberty A, Priem B, Chazalet V, Heyraud A, Joziasse DH, Geremia RA: Production of recombinant xenotransplantation antigen in Escherichia coli. Biochemical and Biophysical Research Communications. 2003, 302: 620-624. 10.1016/S0006-291X(03)00227-4.View ArticleGoogle Scholar
- Mao Z, Shin H-D, Chen RR: Engineering the E. coli UDP-glucose synthesis pathway for oligosaccharide synthesis. Biotechnology Progress. 2006, 22: 369-374. 10.1021/bp0503181.View ArticleGoogle Scholar
- Priem B, Gilbert M, Wakarchuk WW, Heyraud A, Samain E: A new fermentation process allows large-scale production of human milk oligosaccharides by metabolically engineered bacteria. Glycobiology. 2002, 12 (4): 235-240. 10.1093/glycob/12.4.235.View ArticleGoogle Scholar
- Endo T, Koizumi S, Tabata K, Ozaki A: Large-scale production of CMP-NeuAc and sialylated oligosaccharides through bacterial coupling. Applied Microbiology & Biotechnology. 2000, 53: 257-261. 10.1007/s002530050017.View ArticleGoogle Scholar
- Dumon C, Priem B, Martin SL, Heyraud A, Bosso C, Samain E: In vivo fucosylation of lacto-N-neotetraose and lacto-N-neohexaose by heterologous expression of Helicobacter pylori α-1,3 fucosyltransferase in engineered Escherichia coli. Glycoconjugate Journal. 2001, 18: 465-474. 10.1023/A:1016086118274.View ArticleGoogle Scholar
- Koizumi S, Endo T, Tabata K, Nagano H, Ohnishi J, Ozaki A: Large-scale production of GDP-fucose and Lewis X by bacterial coupling. Journal of Industrial Microbiology & Biotechnology. 2005, 25: 213-217.View ArticleGoogle Scholar
- Mattila P, Rabina J, Hortling S, Helin J, Renkonen R: Functional expression of Escherichia coli enzymes synthesizing GDP-L-fucose from inherent GDP-D-mannose in Saccharomyces cerevisiae. Glycobiology. 2000, 10 (10): 1041-1047. 10.1093/glycob/10.10.1041.View ArticleGoogle Scholar
- Cottaz S, Samain E: Genetic engineering of Escherichia coli for the production of NI, NII-diacetylchitobiose (chitinbiose) and its utilization as a primer for the synthesis of complex carbohydrates. Metabolic Engineering. 2005, 7: 311-317. 10.1016/j.ymben.2005.05.004.View ArticleGoogle Scholar
- Antoine T, Priem B, Heyraud A, Greffe L, Gilbert M, Wakarchuk WW, Lam JS, Samain E: Large-Scale in vivo synthesis of the carbohydrate moieties of gangliosides GM1 and GM2 by metabolically engineered Escherichia coli. ChemBioChem. 2003, 4: 406-412. 10.1002/cbic.200200540.View ArticleGoogle Scholar
- Bettler E, Samain E, Chazalet V, Bosso C, Heyraud A, Joziasse DH, Wakarchuk WW, Imberty A, Geremia RA: The living factory: In vivo production of N-acetyllactosamine containing carbohydrates in E. coli. Glycoconjugate Journal. 1999, 16: 205-212. 10.1023/A:1007024320183.View ArticleGoogle Scholar
- Ringenberg M, Lichtensteiger C, Vimr E: Redirection of sialic acid metabolism in genetically engineered Escherichia coli. Glycobiology. 2001, 11 (7): 533-539. 10.1093/glycob/11.7.533.View ArticleGoogle Scholar
- Ruffing A, Mao Z, Chen RR: Metabolic engineering of Agrobacteriumsp. for UDP-galactose regeneration and oligosaccharide synthesis. Metabolic Engineering. 2006, Google Scholar
- Yamada T, Kawasaki T: Microbial synthesis of hyaluronan and chitin: New approaches. Journal of Bioscience and Bioengineering. 2005, 99 (6): 521-528. 10.1263/jbb.99.521.View ArticleGoogle Scholar
- Widner B, Behr R, Von Dollen S, Tang M, Heu T, Sloma A, Sternberg D, DeAngelis PL, Weigel PH, Brown S: Hyaluronic acid production in Bacillus subtilis. Applied and Environmental Microbiology. 2005, 71 (7): 3747-3752. 10.1128/AEM.71.7.3747-3752.2005.View ArticleGoogle Scholar
- Blank LM, McLaughlin RL, Nielsen LK: Stable production of hyaluronic acid in Streptococcus zooepidemicus chemostats operated at high dilution rate. Biotechnology and Bioengineering. 2005, 90 (6): 685-693. 10.1002/bit.20466.View ArticleGoogle Scholar
- Chong BF, Blank LM, Mclaughlin R, Nielsen LK: Microbial hyaluronic acid production. Applied Microbiology & Biotechnology. 2005, 66 (4): 341-351. 10.1007/s00253-004-1774-4.View ArticleGoogle Scholar
- Ertesvag H, Hoidal HK, Schjerven H, Svanem BI, Valla S: Mannuronan C-5-epimerases and their application for in vitro and in vivo design of new alginates useful in biotechnology. Metabolic Engineering. 1999, 1 (3): 262-269. 10.1006/mben.1999.0130.View ArticleGoogle Scholar
- van Kranenburg R, Boels IC, Kleerebezem M, de Vos WM: Genetics and engineering of microbial exopolysaccharides for food: Approaches for the production of existing and novel polysaccharides. Current Opinion in Biotechnology. 1999, 10 (5): 498-504. 10.1016/S0958-1669(99)00017-8.View ArticleGoogle Scholar
- Lee J-h, Lee IY: Optimization of uracil addition for curdlan (β-1→3-glucan) production by Agrobacterium sp. Biotechnology Letters. 2001, 23: 1131-1134. 10.1023/A:1010516001444.View ArticleGoogle Scholar
- Boels IC, Ramos A, Kleerebezem M, de Vos WM: Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar nucleotide and exopolysaccharide biosynthesis. Applied and Environmental Microbiology. 2001, 67 (7): 3033-3040. 10.1128/AEM.67.7.3033-3040.2001.View ArticleGoogle Scholar
- Thorne L, Mikolajczak MJ, Armentrout RW, Pollock TJ: Increasing the yield and viscosity of exopolysaccharides secreted by Sphingomonas by augmentation of chromosomal genes with multiple copies of cloned biosynthetic genes. Journal of Industrial Microbiology & Biotechnology. 2000, 25: 49-57. 10.1038/sj.jim.7000019.View ArticleGoogle Scholar
- Levander F, Svensson M, Radstrom P: Enhanced exopolysaccharide production by metabolic engineering of Streptococcus thermophilus. Applied and Environmental Microbiology. 2002, 68 (2): 784-790. 10.1128/AEM.68.2.784-790.2002.View ArticleGoogle Scholar
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