Structural genomics of human proteins – target selection and generation of a public catalogue of expression clones
© Büssow et al; licensee BioMed Central Ltd. 2005
Received: 23 May 2005
Accepted: 05 July 2005
Published: 05 July 2005
The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics initiative, targets human proteins for structure determination. It has implemented high throughput procedures for all steps from cloning to structure calculation. This article describes the selection of human target proteins for structure analysis, our high throughput cloning strategy, and the expression of human proteins in Escherichia coli host cells.
Results and Conclusion
Protein expression and sequence data of 1414 E. coli expression clones representing 537 different proteins are presented. 139 human proteins (18%) could be expressed and purified in soluble form and with the expected size. All E. coli expression clones are publicly available to facilitate further functional characterisation of this set of human proteins.
The Protein Structure Factory
The Protein Structure Factory (PSF) is a joint endeavour of universities, research institutes and companies from the Berlin area [1, 2]. It takes part in the international structural genomics initiative [3, 4] and aims at the determination of human protein structures by X-ray diffraction methods and NMR spectroscopy using standardised high-throughput procedures. A complete pipeline has been established for this purpose that comprises cloning, protein expression in small and large scale, biophysical protein characterisation, crystallisation, X-ray diffraction and structure calculation.
It is known that eukaryotic proteins are often difficult to express in Escherichia coli . Only a certain fraction of these proteins can be overproduced in E. coli in sufficient yield without formation of inclusion body aggregates or proteolytic degradation. Alternative expression systems include cell cultures of various eukaryotic organisms and cell-free, in vitro protein expression. These systems have been greatly improved since 1999, when the PSF project was initiated. In the meantime, E. coli [5–7] and wheat germ in vitro protein synthesis is routinely used by structural genomics projects. At the PSF, yeast expression hosts, Saccharomyces cerevisiae and Pichia pastoris, were successfully established as alternative systems to E. coli, as described in detail previously [9–11]. We will focus here on the results obtained with the E. coli expression system.
E. colistrains and vectors
The T7 RNA polymerase-dependent E. coli expression vector system (pET-vectors) is a universal system to generate recombinant protein for structural analysis . pET vectors are usually combined with the E. coli B strain BL21 and derivatives that are engineered to carry the T7 RNA polymerase gene. These strains, however, have limitations in cloning and stable propagation of the expression constructs. Expression vectors which are regulated by the lac operator are independent of the host strain. Recombination-deficient E. coli K-12 strains are suitable for cloning because of their high transformation rates and because they allow for stable propagation of recombinant constructs. The strain SCS1 (Stratagene; hsdR17(rK- mK+) recA1 endA1 gyrA96 thi-1 relA1 supE44) was found to perform well at the PSF in cloning experiments. It grows relatively fast and allows for robust protein expression.
Codon usage has a major influence on protein expression levels in E. coli , and eukaryotic sequences often contain codons that are rare in E. coli. Especially the arginine codons AGA and AGG lead to low protein yield . This can be alleviated by introducing genes for overexpression of the corresponding tRNAs into the E. coli host cells. We have used the plasmid pSE111 (Figure 1) carrying the argU gene for this purpose. pSE111 is compatible with pQTEV and other common expression vectors. It carries the lacIQ gene for overexpression of the Lac repressor, which is required when using promoters regulated by lac operators. pSE111 was used at the PSF in combination with the expression vectors pQStrep2 and pGEX-6P-1. Strains for overexpression of rare tRNAs are available from Invitrogen (BL21 Codon Plus) and Novagen (Rosetta). The Rosetta strain contains the chloramphenicol-resistant pRARE plasmid that supplies tRNAs for the codons AUA, AGG, AGA, CUA, CCC, GGA . This plasmid is used at the PSF in combination with pQTEV and pGEX-6P-2.
The Additional file 1 psfClones.xml lists the vector and helper plasmid for overexpression of rare tRNAs that was used for each individual clone.
Selection of target proteins
We selected target proteins with higher-than-average chances of successful expression in E. coli and crystallisation . Proteins were excluded for which sequence analysis predicted that structure determination would be difficult. Starting from the complete set of known human proteins, potentially difficult target proteins and proteins of known structure were excluded according to the following criteria:
Since very large proteins are often difficult to express, the maximal length of target proteins was set to 500 amino acids.
Protein regions that are unstructured or only partially structured  may lead to difficulties during protein expression and purification. Unstructured regions are susceptible to proteolyic attack, and represent an obstacle to protein crystallisation. A large proportion of intrinsically unstructured protein sequences are characterised by sequence stretches of low complexity and tandem repeats . Proteins with low complexity regions of more than 20 amino acids length, detected by the SEG program, or with more than one region were excluded [24, 25].
Coiled-coil proteins were excluded from our target list, since this fold is not novel, and structural analysis of coiled coils requires special attention. Many coiled coil proteins form hetero-complexes with other coiled-coil proteins and cannot be studied without their binding partner. Coiled-coils domains are long, extended structures which can usually only be crystallised as domains, i.e. expression constructs lacking other domains have to be prepared. To identify coiled-coil proteins, the program COILS was used [26–28].
The cellular localisation of target proteins was assigned with the Meta_A(nnotator) [29, 30]. Target proteins annotated to be localised in the extracellular space, endoplasmic reticulum, Golgi stack, peroxisome or mitochondria were excluded from expression in E. coli. Many of these proteins require formation of disulphide bonds for correct folding, but these are generally not formed in the reducing environment of the E. coli cytoplasm. Therefore, these proteins were allocated only for extracellular expression by yeast host cells. Proteins with predicted intracellular localisation or which were not assigned with a localisation by Meta_A(nnotator) were expressed in the cytosol of E. coli.
Potential target proteins were matched to the sequences of proteins with known structure at the Protein Data Bank (PDB) . PSI-BLAST  was used to detect even very distinct homologies to PDB entries to rule out proteins with known folds. This filter was later replaced by a less stringent one, which considers the sequence identity and 'coverage' of matches to PDB sequences. The coverage is the length of the sequence match divided by the protein length. According to the less stringent filter, proteins with 60% or more sequence identity over 90% or more of the sequence length were excluded. Thereby target proteins could be included of which only a part, e.g. a single domain, has a known structure.
The first target list was generated in 1999, when the PSF project started, from the set of all human proteins known at that time. This set was filtered as described above. PSI-BLAST was used to match potential target proteins to the PDB and to include only proteins of presumably novel folds. To enable high-throughput cloning, we selected target proteins for which full-length cDNA clones were available. In 1999, cDNA clones of the IMAGE consortium represented the main public source of sequenced cDNA clones . However, only partial sequence information existed for these clones – the EST sequences of the dbEST database  – and only a small proportion contained complete open reading frames (full-ORF clones). 490 proteins were selected which met the selection criteria, had no match to PDB detected by PSI-BLAST and for which full-ORF IMAGE cDNA clones were available.
In 2003, a second target list was compiled from novel full-length cDNAs discovered by the German cDNA consortium [35, 36]. The same filter criteria as for the first target list were applied, except that cellular localisation was not taken into account. Proteins with a PDB match of 60% or more sequence identity and 90% sequence coverage were excluded, resulting in a target list of 259 proteins.
A third set of target proteins was selected from a human cDNA expression library (hEx1), which was cloned in a bacterial expression vector. This library was screened for expression clones on high density arrays and by high-throughput protein expression and purification experiments [37–40]. This identified 2,700 clones expressing soluble His-tag fusion proteins that could be purified by affinity chromatography . The cDNA inserts of these clones were sequenced and assigned to sequences of the Ensembl database . 141 proteins represented by clones of the expression library where selected as targets for structural analysis . These clones express soluble, full-length proteins, of which the three-dimensional structure was unknown.
Origin of template cDNA clones Numbers of targets grouped by type of template cDNA clone
Number of targets
Number of successfully cloned cDNA
Number of proteins with soluble expression
Number of structures
1 – IMAGE clones
2 – cDNA consortium
3 – hEx1 library
Generation and characterisation of expression clones
We established a common cloning strategy that allows for easy shuttling of cDNA fragments between different E. coli and yeast vectors. We adopted a cloning system that adds only a minimal number of extra amino acids to the protein of interest and therefore decided to clone with restriction enzymes instead of using alternative systems, such as Invitrogen's Gateway system or ligation independent cloning .
The PSF has been working with more than a thousand target proteins to date. Suitable cDNA clones were selected and subcloned into the E. coli expression vectors pQTEV GenBank AY243506 and pQStrep2 AY028642. These vectors provide for an N-terminal His-tag; pQStrep2 also encodes an C-terminal Strep-tag-II. Some proteins have also been expressed as GST fusion proteins using the vectors pGEX-4T2 or pGEX-6P1 (Amersham Biosciences).
Expression of protein coding genes from multiple transformants per target was tested under multiple conditions. Standardisation and automation was introduced to achieve this throughput. Expression clones were characterised by small scale protein synthesis at different temperatures, 37°C, 30°C and 25°C, in 1 ml volumes in deep-96-well microplates. Proteins were purified in parallel by a pipetting robot, as described previously . 10% of the purified protein eluate from a 1 ml culture was analysed by SDS-PAGE. For each protein expression experiment, the size of the expression product was recorded and the amount of protein was classified into four categories: none, weak, moderate and strong expression. This classification is arbitrary to a certain degree, however, we found it sufficient to select suitable clones for protein production scale-up.
PSF E. coli expression clones with soluble expression products. The table corresponds to the proteins shown in Figure 2. More detailed information is available in the supplementary XML file, Additional file 1.
NCBI Entrez gene ID
Gene symbol, name
RZPD clone ID
Sequence verified, non-silent mutations
Protein size [Da]
Protein gel, lane
ACAT2, acetyl-Coenzyme A acetyltransferase 2
AK1, adenylate kinase 1
BTF3, basic transcription factor 3
CAPZA2, capping protein (actin filament) muscle Z-line, alpha 2
CDO1, cysteine dioxygenase, type I
CRYM, crystallin, mu
CSNK2B, casein kinase 2, beta polypeptide
DGKA, diacylglycerol kinase, alpha 80kDa
DBN1, drebrin 1
DCTD, dCMP deaminase
EEF1G, eukaryotic translation elongation factor 1 gamma
EIF4A2, eukaryotic translation initiation factor 4A, isoform 2
GTF2F2, general transcription factor IIF, polypeptide 2, 30 kDa
HMGN2, high-mobility group nucleosomal binding domain 2
HSPA8, heat shock 70 kDa protein 8
KARS, lysyl-tRNA synthetase
STMN1, stathmin 1/oncoprotein 18
LRPAP1, low density lipoprotein receptor-related protein associated protein 1
NDUFA2, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2
NDUFA5, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5
PEPD, peptidase D
PFDN2, prefoldin 2
UBL3, ubiquitin-like 3
PPP1R1A, protein phosphatase 1, regulatory (inhibitor) subunit 1A
PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin)
PSMD11, proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
RABIF, RAB interacting factor
RPL9, ribosomal protein L9
RPL30, ribosomal protein L30
RPS4X, ribosomal protein S4, X-linked
SCP2, sterol carrier protein 2
SH3BGRL, SH3 domain binding glutamic acid-rich protein like
SRP19, signal recognition particle 19 kDa
TALDO1, transaldolase 1
TCTE1L, t-complex-associated-testis-expressed 1-like
TCTEL1, t-complex-associated-testis-expressed 1-like 1
PRDX2, peroxiredoxin 2
TPT1, tumor protein, translationally-controlled 1
UFD1L, ubiquitin fusion degradation 1-like
UROS, uroporphyrinogen III synthase
XRCC4, X-ray repair complementing defective repair in Chinese hamster cells 4
YWHAE, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
ANP32A, acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
DGCR6, DiGeorge syndrome critical region gene 6
TAGLN2, transgelin 2
PRKRA, protein kinase, interferon-inducible double stranded RNA dependent activator
STX10, syntaxin 10
SNX3, sorting nexin 3
G10, maternal G10 transcript
SNURF, SNRPN upstream reading frame
AIP, aryl hydrocarbon receptor interacting protein
FIBP, fibroblast growth factor (acidic) intracellular binding protein
TMSB10, thymosin, beta 10
CNOT8, CCR4-NOT transcription complex, subunit 8
GGPS1, geranylgeranyl diphosphate synthase 1
AKAP7, A kinase (PRKA) anchor protein 7
PHYHIP, phytanoyl-CoA hydroxylase interacting protein
AKT3, v-akt murine thymoma viral oncogene homolog 3
COX17, COX17 homolog, cytochrome c oxidase assembly protein (yeast)
ARPC3, actin related protein 2/3 complex, subunit 3
SERF2, small EDRK-rich factor 2
STX6, syntaxin 6
HRSP12, heat-responsive protein 12 (14.5 kDa translational inhibitor protein, p14.5)
APEG1, aortic preferentially expressed protein 1
TXNL2, thioredoxin-like 2
MTHFS, 5,10-methenyltetrahydrofolate synthetase
DRAP1, DR1-associated protein 1 (negative cofactor 2 alpha)
AHSA1, AHA1, activator of heat shock 90 kDa protein ATPase homolog 1 (yeast)
PAICS, phosphoribosylaminoimidazole carboxylase
C7orf16, chromosome 7 open reading frame 16
RUVBL2, RuvB-like 2 (E. coli)
GADD45G, growth arrest and DNA-damage-inducible, gamma
MORF4L1, mortality factor 4 like 1
STIP1, stress-induced-phosphoprotein 1
CDC37, CDC37 cell division cycle 37 homolog (S. cerevisiae)
COPE, coatomer protein complex, subunit epsilon
PDAP1, PDGFA associated protein 1
GABARAP, GABA(A) receptor-associated protein
PTK9L, PTK9L protein tyrosine kinase 9-like (A6-related protein)
COPZ1, coatomer protein complex, subunit zeta 1
MAPRE1, microtubule-associated protein, RP/EB family, member 1
RAB18, RAB18, member RAS oncogene family
CARHSP1, calcium regulated heat stable protein 1
ASF1A, ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
PREI3, preimplantation protein 3
DKFZP566E144, small fragment nuclease
AK5, adenylate kinase 5
yes, 3 bp insertion at 3' end
HSPB8, heat shock 22 kDa protein 8
TRAPPC3, trafficking protein particle complex 3
C2orf25, chromosome 2 open reading frame 25
eIF3k, eukaryotic translation initiation factor 3 subunit 12
MAT2B, methionine adenosyltransferase II, beta
PTD004, GTP-binding protein PTD004
KCNIP2, Kv channel interacting protein 2
CUTC, cutC copper transporter homolog (E. coli), CGI-32
THAP4, THAP domain containing 4
HN1, hematological and neurological expressed 1
yes, 3 bp deletion at 5' end, G457A
VPS28, vacuolar protein sorting 28 (yeast)
NEUGRIN, mesenchymal stem cell protein DSC92
COMMD10, COMM domain containing 10
ANAPC5, anaphase promoting complex subunit 5
LCMT1, leucine carboxyl methyltransferase 1
C6orf55, chromosome 6 open reading frame 55
MPP6, membrane protein, palmitoylated 6
SUHW4, suppressor of hairy wing homolog 4 (Drosophila)
WDR41, WD repeat domain 41
PGM2, phosphoglucomutase 2
SARA1, SAR1a gene homolog 1 (S. cerevisiae)
C21orf7, chromosome 21 open reading frame 7
LOC57019, cytokine induced apoptosis inhibitor 1
TRAPPC1, trafficking protein particle complex 1
RAB17, RAB17, member RAS oncogene family
C6orf60, chromosome 6 open reading frame 60
PLEKHF2, pleckstrin homology domain containing, family F member 2
FBXO31, F-box protein 31
COASY, Coenzyme A synthase
ILKAP, integrin-linked kinase-associated serine/threonine phosphatase 2C
RAB1B, RAB1B, member RAS oncogene family
DKFZP566J2046, fumarylacetoacetate hydrolase domain containing 1
DKFZP434H0115, hypothetical protein DKFZp434H0115
C9orf58, chromosome 9 open reading frame 58
SESN2, sestrin 2
NOHMA, HORMA domain containing protein
CIP29, cytokine induced protein 29 kDa
PHYHIPL, phytanoyl-CoA hydroxylase interacting protein-like
MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha
MGC20398, hypothetical protein MGC20398
EPSTI1, epithelial stromal interaction 1 (breast)
RWDD2, RWD domain containing 2
C10orf83, chromosome 10 open reading frame 83
FLJ30046, hypothetical protein FLJ30046
C20orf79, chromosome 20 open reading frame 79
Biophysical properties of proteins which could be expressed in soluble form in E. coli were compared against all tested proteins. We found no significant correlation between expression success and either protein length or mean net charge (data not shown). However, when analysing the mean hydrophobicity, we found that hydrophobic proteins are less likely to be expressed in soluble form. Only one of 139 well expressed proteins has a mean hydrophobicity of more than 0.2, while 8% of the other proteins are above this value. This group of proteins does not contain transmembrane helices according to TMHMM, and therefore may represent peripheral membrane proteins with hydrophobic surface regions.
Gene ID and name,
Strain and vector,
Protein expression results,
Novel human protein structures The structures of full length proteins solved by the Protein Structure Factory.
NCBI Entrez gene ID
GenBank protein accession
APEG1, aortic preferentially expressed protein 1
Fumarylacetoacetate hydrolase family member FLJ36880
14.5 kDa translational inhibitor protein, p14.5
BET3, trafficking protein particle subunit
CutC copper transporter homolog, CGI-32
Nicotinamide mononucleotide adenylyltransferase
We describe here the strategies and experiments of our structural genomics project on human proteins. In addition to the expression of full length proteins, the Protein Structure Factory has also studied protein domains by NMR spectroscopy, which has been described elsewhere [45–47]. Our selection of full length target proteins was mainly determined by the availability of full length cDNA clones. In addition, biophysical and bioinformatical criteria were applied, leading to a biased selection of target proteins from the human proteome. Therefore, we expect that the percentage of proteins that we could express and purify in soluble form, 18%, is higher than it would be in a randomly selected set. The low proportion of successfully expressed proteins indicates that E. coli is not the appropriate expression host for many full length human proteins. High throughput protein expression in alternative system such as yeast [9–11] or insect cells/baculovirus  has been established and will lead to better success rates in future projects.
Generally, clones that did express a soluble protein were verified by DNA sequencing, while clones that did not express or expressed an insoluble product were usually not sequence verified. It cannot be ruled out that some of the unsuccessful clones contain sequence errors introduced during cloning. Since template cDNA clones of the IMAGE consortium with only partial sequence information were used for most cloning experiments, expression clones that were not sequence verified might represent splice variants or isoforms of the original target. The distribution of mean net charge and length was similar among successfully expressed and all proteins, while very hydrophobic proteins were generally not expressed well in our E. coli expression system.
Future efforts in structural genomics of mammalian proteins will benefit from a much better supply of full length cDNA clones. Clones prepared for protein expression by resource centres and commercial suppliers are becoming available now. With such resources, alternative target selection strategies will become feasible that will not be restricted by the availability of cDNA clones. Instead, all potential target proteins, including splice variants, could be clustered by similarity and the most suitable members of each cluster could be selected by appropriate criteria as outlined in the Background section.
In our approach, we have excluded certain types of proteins such as membrane proteins and very large proteins. A structural genomics approach that includes membrane proteins would require standard protocols to optimise expression conditions and detergents . The best strategy to study large proteins is to divide them into domains and smaller regions. However, such smaller constructs usually have to be designed manually.
All clones listed in the supplementary file (Additional file 1) and Table 2 are available to the research community. Thereby we hope to facilitate further functional characterisation of this set of human proteins.
Materials and Methods
Cloning with restriction enzymes
cDNA inserts were amplified by PCR primers carrying tails with BamHI and NotI sites and cloned into the respective sites of one of the expression vectors pQTEV, pQStrep2 or pGEX-6p1. This had the drawback that the restriction sites chosen for cloning might occur in the insert. In such cases, compatible overhangs were produced by alternative enzymes or by the hetero-stagger cloning method . Alternative enzymes are BglII for BamHI and the type IIs enzymes BpiI, Eco31I, Esp3I, which can replace both BamHI and NotI. Type IIs enzymes cut outside their recognition sequence and can produce arbitrary overhangs.
PCR Primer design
PCR primers with tails carrying restriction enzyme cleavage sites were designed automatically by a Perl program. The primer design program adjusts the length of the primers to achieve a melting temperature close to a common default. Then, restriction enzymes that do not cut within the respective cDNA sequence are selected by the program and restriction enzyme sites are attached to the primer sequences. Finally, since restriction enzymes do not cut well at the very end of a DNA molecule, an additional short nucleotide tail is automatically attached to the primers. The sequence of this tail is optimised to minimise formation of secondary structure, hairpins or dimerisation. A Java version of the primer design software, 'ORFprimer', is publicly available .
Automated high-throughput cloning, protein expression and purification
PCR primers and cDNA clones were delivered in 96-well microplate format. Upon delivery, plates with PCR primers and template clones were reformatted to obtain corresponding plate positions by a Zinsser Speedy pipetting robot. The PCR master mix (Roche Expand) and cDNA primers (10 μM stocks) were pipetted into a PCR microplate with a multichannel pipet. Template clone bacteria were added with a 96-pin steel replicating device from overnight cultures in microtitre plates. PCR product size and yield was determined by agarose gel electrophoresis and the software Phoretix 1D Quantifier (Nonlinear dynamics). PCR products were purified with magnetic beads on the pipetting robot with a system that has been developed at the Max Planck Institute of Molecular Genetics in collaboration with Bruker Daltonics (Bruker genopure kit). The correct restriction enzyme master mixes were automatically added and the digested fragments were purified again, analysed by agarose gel electrophoresis and quantified. The robot then adjusted DNA concentrations to a common default by dilution. Ligations were set up manually with a multichannel pipet, and SCS1 E. coli cells carrying pRARE were transformed in a PCR microplate by chemical transformation on a PCR machine . Transformed cells were manually plated on individual agar plates. Four clones were picked per transformation and were checked by PCR using vector primers. E. coli expression clones were ready for protein expression at this stage.
Primer sequences and template clones for cloning of the target cDNAs are listed in the supplementary XML file, Additional file 1.
The characterisation of expression clones by parallel expression and protein purification is described in reference .
Sequence analysis software was run with default settings unless indicated otherwise. The mean charge of a protein was calculated as the difference of the number of positive and negatively charged amino acids (Lys, Arg and Glu, Asp, respectively) divided by the protein length. The mean hydrophobicity was calculated with the Kyte and Doolittle hydropathy index , obtained from the EMBOSS package . The index values were added up for a given protein and divided by the protein length.
We wish to thank Ulf Lenski for help with sequence analysis and Janett Tischer for technical assistance. This work was funded by the German Federal Ministry of Education and Research (BMBF) through the Leitprojektverbund Proteinstrukturfabrik and the National Genome Network NGFN (FKZ 01GR0471, 01GR0472) and with support by the Fonds der Chemischen Industrie to U.H. Support by the Berlin Senate and the European Fund for Regional Development (EFRE) is also gratefully acknowledged.
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