Gold nanoparticles synthesized by Geobacillus sp. strain ID17 a thermophilic bacterium isolated from Deception Island, Antarctica
- Daniela N Correa-Llantén†1Email author,
- Sebastian A Muñoz-Ibacache†1, 2Email author,
- Miguel E Castro†1,
- Patricio A Muñoz†1, 3Email author and
- Jenny M Blamey†1, 3Email author
© Correa-Llantén et al.; licensee BioMed Central Ltd. 2013
Received: 13 May 2013
Accepted: 31 July 2013
Published: 6 August 2013
The use of microorganisms in the synthesis of nanoparticles emerges as an eco-friendly and exciting approach, for production of nanoparticles due to its low energy requirement, environmental compatibility, reduced costs of manufacture, scalability, and nanoparticle stabilization compared with the chemical synthesis.
The production of gold nanoparticles by the thermophilic bacterium Geobacillus sp. strain ID17 is reported in this study. Cells exposed to Au3+ turned from colourless into an intense purple colour. This change of colour indicates the accumulation of intracellular gold nanoparticles. Elemental analysis of particles composition was verified using TEM and EDX analysis. The intracellular localization and particles size were verified by TEM showing two different types of particles of predominant quasi-hexagonal shape with size ranging from 5–50 nm. The mayority of them were between 10‒20 nm in size. FT-IR was utilized to characterize the chemical surface of gold nanoparticles. This assay supports the idea of a protein type of compound on the surface of biosynthesized gold nanoparticles. Reductase activity involved in the synthesis of gold nanoparticles has been previously reported to be present in others microorganisms. This reduction using NADH as substrate was tested in ID17. Crude extracts of the microorganism could catalyze the NADH-dependent Au3+ reduction.
Our results strongly suggest that the biosynthesis of gold nanoparticles by ID17 is mediated by enzymes and NADH as a cofactor for this biological transformation.
KeywordsGeobacillus Nanoparticles Gold Deception island Antarctica
Biological synthesis of nanoparticles appears as a suitable process since it requires less energy, is environmentally safe[1, 2], it has low manufacture costs of scalability, and better nanoparticle stabilization, compared to chemically synthesized nanoparticles[3, 4].
Nanoparticles have large surface to volume ratio, thus surface related phenomena and properties are drastically affected with slight modification of size, shape and surrounding media. Therefore, the desired optical properties of nanoparticles, depending on the application, can be tuned by generating nanoparticles of defined size and shape in selected media allowing the development of new effective nanomaterials and nanodevices.
Gold nanoparticles show very high chemical reactivity compared to bulk gold, well known for being inert. This kind of nanoparticles has multiple applications in drug-delivery, gene transfer, as bioprobes in cells and for tissue analysis in visualization of micro- and nano-objects, for observation of biological processes at nanoscale[6, 7] to enhance electroluminescence and quantum efficiency in organic light emitting diodes.
Biosynthesis of gold nanoparticles has been reported in different prokaryotic organisms including Bacillus subtilis, Escherichia coli, Lactobacillus, Pseudomonas aeruginosa, Rhodopseudomonas capsulata, but the molecular mechanisms involved in the metal ion reduction taking place for the synthesis of nanoparticles has not yet been established.
Some of these microorganisms can survive and grow even at high metal ion concentrations. They are often exposed to extreme environmental conditions, which forces them to develop specific defense mechanisms to quell such stresses, including the toxicity of foreign metal ions or metalloids.
For these reasons and with an applied view we search for microorganisms resistant to high metal concentrations in Deception Island, Antarctica. This place is a complex stratovolcano with a “horseshoe” shape whose central part has a caldera structure. This volcanic island has been very active during the last century: fumarolic emissions, thermal springs and hot soils are evidence of Deception Island’s continuing activity, making it an interesting site for searching new thermophilic bacteria.
Here, we report the synthesis of metallic gold nanoparticles by Geobacillus sp. strain ID17 mediated by NADH-dependent enzymes that reduce Au3+ to elemental gold. Cells exposed to Au3+ turned from colourless to an intense purple colour. This change in colour indicates intracellular gold nanoparticles accumulation.
Results and discussion
The intracellular biosynthesis of gold nanoparticles by a thermophilic bacterium ID17 belonging to genus Geobacillus strain isolated from Deception Island, was carried out using whole living bacterial cells and cell-free lysates.
Reductase activity has been previously reported to be present in other microorganisms involved in nanoparticles synthesis, where the reduction might be initiated by the electron transfer from NADH by a NADH-dependent reductase allowing the reduction of Au3+. Similar process has been described for selenium reduction, where the biogenesis of selenium nanoparticles by Bacillus cereus involves membrane associated reductases that reduces selenite to elemental selenium through electron shuttle enzymatic metal reduction process. However, the molecular mechanism involved in the reduction of cationic gold is still unclear.
Regarding nanoparticles form, the quasi-hexagonal crystal shape is the predominant form under the experimental conditions reported in this work. Studies reveal that the morphology and dimensions of nanoparticles are strongly dependent of biosynthesis conditions, such as temperature, concentration of metallic salt, pH. He et al. demonstrated that the bacterium Rhodopseudomonas capsulate produces gold nanoparticles of different sizes and shapes when was incubated with HAuCl4 salts, was exposed to distinct pH values (spherical at pH 7.0 and nanoplates at pH 4.0), converting it in the most important factor to control these parameters. We think that the influence of different reductases associated to the biosynthesis could result in differences of shape but our experimental results cannot be conclusive to probe this hypothesis.
Gold nanoparticles shape and size are very important factors for the immunological response in vitro and in vivo. It has been reported that 40 nm spherical nanoparticles induce the highest level of specific antibodies against West Nile virus, while rod nanoparticles lead only to 50% of the antibody production against the virus, indicating the high variability of immunological responses when different nanoparticle shapes were used.
Moreover, gold nanoparticles have different biological applications that include immunostaining of specific molecules or compartments of cells by antibodies. Using the previous concept, gold nanoparticles can be useful as contrast agents for X-rays. They also can be used as vehicle for delivery of molecules into cells, where the molecules are absorbed on the surface of gold nanoparticles and the whole conjugate is introduced into the cells. Finally the intracellular accumulation of gold nanoparticles by Geobacillus sp. provides also a potential application of this microorganism in bioremediation of gold-bearing waste. Even more, gold nanoparticles can be used as a heat source, if they absorb light by their inner electrons and dissipating heat when they relax.
ID17, a thermophilic bacterium belonging to the genus Geobacillus, has the ability to biosynthesize gold nanoparticles, which are intracellularly accumulated. This property is present in whole cells and in free cell extracts indicating that this process is probably enzymatically mediated, due to the requirement of NADH as cofactor for this biological transformation.
Bacterial strains and culture conditions
ID17 was isolated as described by Muñoz et al. (2011) from environmental samples from Deception Island (Antarctica). Cells were grown in BS medium (0.3 g/L NaCl; yeast extract 0.15 g/L; 0.3 g/L tryptone) for 16 h at 65°C and pH 7.0. The sequence of the 16S rDNA of the isolate was deposited in Genbank nucleotide sequences databank under accession number U366067.
Biosynthesis of gold nanoparticles
Assays were carried out using whole living bacterial cells and cell-free lysates. For the biosynthesis of gold nanoparticles, cells from culture with OD600 ~ 0,5 were harvested by centrifugation at 6,000 rpm for 5 min and washed three times with sterile water.
Using whole living bacterial cells assay
The harvested bacteria were suspended in 10 mL solution of 20 mM potassium phosphate buffer, pH 7.0 containing 1 mM hydrogen tetrachloroaurate (HAuCl4 × 3H2O, Sigma). The solution was incubated at 65°C for 16 h. Bacterial-gold nanoparticles were recovered as described by Fesharaki et al.,. Cell debris was discarded and nanoparticles were characterized.
Using cell-free lysates assay
Cell pellet was suspended in 20 mM potassium phosphate buffer, pH 7.0 and disrupted by sonication for 10 min at level 3 in a Sonifier 450 (Branson). Cell debris was discarded and the supernatant was kept at 4°C. Protein concentration was determined as described by Bradford using bovine serum albumin as standard.
Crude extracts were assayed for their ability to reduce hydrogen tetrachloroaurate. To determine the presence of the intracellular enzymes involved in the biological transformation, 100 μg of protein were added in 20 mM potassium phosphate buffer, pH 7.0 containing 1 mM hydrogen tetrachloroaurate and NADH in a range of 0–10 mM. Final volume of reaction was 1 mL. Reactions were always started by the addition of free cell extract and incubated at 65°C for 30 min. The reaction was followed by measuring the change in absorbance at 540 nm in a spectrophotometer.
To identify the purple deposits as reduced Au3+, free cell extract was subjected to non-denaturing polyacrylamide (10%) gel electrophoresis (PAGE) to detect Au3+ reduction activity. The native gel containing the proteins present in the crude extract was immersed in 20 mM potassium phosphate buffer, pH 7.0 containing 1 mM HAuCl4 × 3H2O, 5 mM NADH and incubated at 65°C for 1 h. Reduction activity was detected by the change of colour from colourless to purple.
Gold nanoparticles characterization
Gold nanoparticles spectra were performed scanning from 400 to 900 nm using UV/visible spectrophotometer (Shimadzu UV-1700) and 1.0 cm light-path length cuvette.
Transmission electron microscopy measurements (TEM)
10 μL of sample were dropped on a carbon coated copper grid and dried at room temperature. For the size determination of nanoparticles, bacteria were recovered as described by Fesharaki et al.,. TEM measurements were performed on a Philips Tecnai 12 Bio Twin TEM operating at 200 kV. Sizes were obtained with NIS-Elements D 3.10 software. Histogram distribution of sizes was constructed with Sigma Plot 11.0 software.
Energy-Dispersive X-Ray Microanalysis (EDX)
Elemental analyses of nanoparticles were conducted by using energy- dispersive X- ray microanalysis. This was carried out using a scanning electron microscope (SEM) Jeol 5410 equipped with an energy dispersive X-ray spectrometer.
Fourier-Transform Infrared (FT-IR) Chemical Analysis
For Fourier-Transform Infra-Red spectroscopy measurements, the nanoparticles extracted from bacteria were freeze dried and diluted with potassium bromide in the ratio of 1:100. The FT-IR spectra of samples were recorded on a FT-IR instrument (IFS66V, Bruker). All measurements were carried out in the range of 400–4,000 cm-1 at a resolution of 4.0 cm-1.
This work received financial support from Grant G04-09 from Instituto Antártico Chileno (INACH), Grant Innova - CORFO Nº 07CN13PXT-264, and Air Force Office of Scientific Research (AFOSR), USA, Grant Nº FA9550-13-1-0089.
- He S, Guo Z, Zhang Y, Zhang S, Wang J, Gu N: Biosynthesis of gold nanoparticles using the bacteria Rhodopseudomonas capsulate. Mater Lett. 2007, 61: 3984-3987. 10.1016/j.matlet.2007.01.018.View Article
- Binupriya AR, Sathishkumar M, Yun S: Myco-crystallization of silver ions to nanosized particles by Live and Dead Cell Filtrates of Aspergillus oryzae var. Wiridis and its bactericidal activity toward Staphylococcus aureus KCCM 12256. Ind Eng Chem Res. 2010, 49: 852-858. 10.1021/ie9014183.View Article
- Binupriya AR, Sathishkumar M, Vijaraghavab K, Yun S: Bioreduction of trivalent aurum to nano-crystalline gold particles by active and inactive cells and cell-free extract of Aspergillus oryzae var. viridis. J Hazard Mater. 2010, 177: 539-545. 10.1016/j.jhazmat.2009.12.066View Article
- Sneha K, Sathishkumar M, Mao J, Kwak I, Yun Y: Corynebacterium glutamicum-mediated crystallization of silver ions through sorption and reduction process. Chem Eng J. 2010, 162: 989-996. 10.1016/j.cej.2010.07.006.View Article
- Neeleshwar S, Chen CL, Tsai CB, Chen YY, Chen CC, Shyu SG, Seehra MS: Size-dependent properties of CdSe quantum dots. Phys Rev B. 2005, 71:201307: 1-4.
- Deplanche K, Macaskie LE: Biorecovery of gold by Escherichia coli and Desulfovibrio desulfuricans. Biotechnol Bioeng. 2008, 99: 1055-1064. 10.1002/bit.21688View Article
- Salata OV: Application of nanoparticles in biology and medicine. J Nanobiotechnol. 2004, 2: 3-9. 10.1186/1477-3155-2-3.View Article
- Park JH, Lim YT, Park OO, Kim JK, Yu JW, Kim YC: Polymer/Gold nanoparticle nanocomposite light-emitting diodes: enhancement of electroluminescence stability and quantum efficiency of blue-light-emitting polymers. Chem Mater. 2004, 16: 688-692. 10.1021/cm0304142.View Article
- Beveridge TJ, Murray RGE: Site of metal deposition in the cell wall of Bacillus subtilis. J Bacteriol. 1980, 141: 876-887.
- Brown S, Sarikaya M, Johnson E: A genetic analysis of crystal growth. J Mol Biol. 2000, 299: 725-735. 10.1006/jmbi.2000.3682View Article
- Nair B, Pradeep T: Coalescence of nanoclusters and formation of submicron crystallites assisted by Lactobacillus strains. Cryst Growth Des. 2002, 2: 293-298. 10.1021/cg0255164.View Article
- Husseiny MI, Abd El-Aziz M, Badr Y, Mahmoud MA: Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa. Spectrochim Acta A. 2007, 67: 1003-1006. 10.1016/j.saa.2006.09.028.View Article
- Silver S: Bacterial resistances to toxic metal ions. Gene. 1996, 179: 9-19. 10.1016/S0378-1119(96)00323-XView Article
- Caselli A, Dos Santos AM, Agusto MR: Gases fumarólicos de la Isla Decepción (Shetland del Sur, Antártida): variaciones químicas y depósitos vinculados a la crisis sísmica de 1999. Rev Asoc Geol Arg. 2004, 59: 291-302.
- Muñoz PA, Flores PA, Boehmwald FA, Blamey JM: Thermophilic bacteria present in a sample from Fumarole Bay, Deception Island. Antarc Sci. 2011, 23: 549-555. 10.1017/S0954102011000393.View Article
- Binupriya A, Sathishkumar M, Yun S: Biocrystallization of silver and gold ions by inactive cell filtrate of Rhizopus stolonifer. Colloid Surface B. 2010, 79: 531-534. 10.1016/j.colsurfb.2010.05.021.View Article
- Sathishkumar M, Sneha K, Won S, Cho C, Kim S, Yun Y: Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloid Surfaces B. 2009, 73: 332-338. 10.1016/j.colsurfb.2009.06.005.View Article
- Sathishkumar M, Mahadevan A, Vijayaraghavan K, Pavagadhi S, Balasubramanian R: Green recovery of gold through biosorption, bio-crystallization and pyro-crystallization. Ind Eng Chem Res. 2010, 49: 7129-7135. 10.1021/ie100104j.View Article
- Sneha K, Sathishkumar M, Lee S, Bae M, Yun Y: Biosynthesis of Au nanoparticles using cumin seed powder extract. J Nanosci Nanotechnol. 2011, 11: 1811-1814. 10.1166/jnn.2011.3414View Article
- Nangia Y, Wangoo N, Goyal N, Shekhawat G, Raman C: A novel bacterial isolate Stenotrophomonas maltophilia as living factory for synthesis of gold nanoparticles. Microb Cell Fact. 2002, 8: 39-View Article
- Ahmad A, Senapati S, Khan MI, Kumar R, Sastri M: Extracellular biosynthesis of monodisperse gold nanoparticles by novel extremophilic actinomycetes, Thermomonospora sp. Langmuir. 2003, 19: 3550-3553. 10.1021/la026772l.View Article
- Castro ME, Molina R, Díaz W, Pichuantes SE, Vásquez CC: The dihydrolipoamide dehydrogenase of Aeromonas caviae ST exhibits NADH-dependent tellurite reductase activity. Biochem Biophys Res Commun. 2008, 375: 91-94. 10.1016/j.bbrc.2008.07.119View Article
- Sun Y, Xia Y: Shape-controlled synthesis of gold and silver nanoparticles. Science. 2002, 298: 2176-2179. 10.1126/science.1077229View Article
- Niikuram K, Matsunaga T, Suzuki T, Kobayashi S, Yamaguchi H, Orba Y, Kawaguchi A, Hasegawa H, Kajino K, Minomiya T, Ijiro K, Sawa H: Gold nanoparticles as a vaccine plataform: influence of size and shape on immunological responses in vitro and in vivo. ACS Nano. 2013, 7: 3926-3938. 10.1021/nn3057005View Article
- Sperling RA, Rivera P, Zhang F, Zenella M, Parak WJ: Biological applications of gold nanoparticles. Chem Soc Rev. 2008, 37: 1896-1908. 10.1039/b712170aView Article
- Fesharaki PJ, Nazari P, Shakibaie M, Rezaie S, Banoee M, Abdollahi M, Shahverdi AR: Biosynthesis of selenium nanoparticles using KlebsielIa pneumoniae and their recovery by a simple sterilization process. Braz J Microbiol. 2010, 41: 461-466. 10.1590/S1517-83822010000200028.View Article
- Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-254. 10.1016/0003-2697(76)90527-3View Article
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