From rumen to industry
© Sauer et al.; licensee BioMed Central Ltd. 2012
Received: 7 September 2012
Accepted: 7 September 2012
Published: 10 September 2012
The rumen is one of the most complicated and most fascinating microbial ecosystems in nature. A wide variety of microbial species, including bacteria, fungi and protozoa act together to bioconvert (ligno)cellulosic plant material into compounds, which can be taken up and metabolized by the ruminant. Thus, the rumen perfectly resembles a solution to a current industrial problem: the biorefinery, which aims at the bioconversion of lignocellulosic material into fuels and chemicals. We suggest to intensify the studies of the ruminal microbial ecosystem from an industrial microbiologists point of view in order to make use of this rich source of organisms and enzymes.
KeywordsRumen Industrial microbiology Lignocellulose conversion Microbial community Microbial ecosystem Metabolic engineering Microbial organic acid production Biofuels
The rumen is a rich source of enzymes for biorefineries
The rumen is a rich source of microbial cell factories
The rumen is a model to study the higher organisational levels of microbial communities, finally leading to a new concept for metabolic engineering
Enzymes for the lignocellulose based biorefinery
The use of lignocellulosic plant material as carbon source for fuel and chemical production is currently one of the focal points of biotechnology . However, the plant cell wall evolved for stability and robustness and not as a carbon storage compound. Consequently, the enzymatic deconstruction of the plant cell wall poses significant challenges due to its chemical and physical complexity. Despite major investments in research and significant advances in technology, this remains one of the critical points for the establishment of economic biorefineries. Generally, the plant material has to be subjected to a pretreatment followed by enzymatic hydrolysis to obtain a microbially accessible carbon source. A wide variety of bacteria and fungi serving as decomposer in the natural carbon cycle secrete industrially useful cellulolytic enzymes .
Shifting our view to ruminants the situation is quite similar. Rumination is the pretreatment of the biomass to render it enzymatically accessible. After rumination the biomass is degraded in the rumen by the complex microbial community and converted into compounds, which are useful for the ruminant. This resembles a modern biorefinery . Also in this case the involved enzymes are of bacterial as well as fungal origin, whereby the bacteria seem to be more important .
A recent example published in this journal highlights the suitablity of the rumen as a playground for the industrial microbiologist: Zheng et al.  set out to engineer E. coli for succinate production from hemicelluloses. In their search for xylanolytic enzymes 3 out of 7 possible enzyme sources were ruminal bacteria. In fact, the combination of one enzyme from Fibrobacter succinogenes, a ruminal bacterium, and another enzyme from Fusarium graminearum, a plant pathogen fungus turned out to have optimal enzymatic properties for xylan hydrolysis.
Microbial cell factories found in the rumen
The rumen community with its peculiar interconnections comprises a plethora of microorganisms, which produce chemicals of interest. Here, nature provides us with a wealth of production hosts, which are only scarcely known up to now. Organic acids are the main chemicals used as currencies which are converted among the microorganisms and the microbial community with their host. Organic acid producers are therefore among the best studied ruminal cell factories so far. A prominent example is the microbial production of succinic acid. In fact, succinate is an important metabolic intermediate in the rumen. Several bacteria obtain energy by decarboxylating succinate to propionate, which in turn serves as a nutrient for the ruminant . Among the identified ruminal succinic acid producers are Anaerobiospirillum succiniproducens Actinobacillus succinogenes, and Mannheimia succiniproducens, just to name a few . The importance of the characteristics of the natural environment for the production process has recently been highlighted in this journal for A. succinogenes by Zou et al. : The main fraction of the gaseous phase in the rumen is CO2. CO2 is also a substrate for phosphoenolpyruvate carboxykinase, a key enzyme for succinic acid biosynthesis. Thus, a significant influence of the CO2 provision on succinic acid production appears plausible. Zuo et al.  could show that not only gaseous CO2 but also the presence of carbonates, such as MgCO3 are beneficial for succinic acid production. The key message here is that the industrial microbiologist should never forget, where his favourite microbial cell factory comes from, when designing the bioprocess.
A second example for an industrially interesting ruminal organic acid producer is Megsphaera elsdenii. M. elsdenii readily accumulates short chain aliphatic organic acids such as butyric, valeric or caproic acids when cultured under appropriate conditions . Particularly, caproic (hexanoic) acid production is of interest as it could open a sustainable production route for Nylon. Recently, we obtained the genome sequence of this organism . Deducing the metabolic pathway for hexanoic acid production we speculate that the production of hexanoic acid serves as an electron sink, enabling M. elsdenii to get rid of reduction equivalents, growing on glucose. Again, one has to consider the anaerobic environment (no oxygen as electron acceptor) and the profound competition for sugars as carbon sources in the rumen. So it appears plausible that this organism seeks to take up as much carbon source as quickly as possible - even on the expense of carbon for electron dissipation. Apart from the peculiar metabolic activities, M. elsdenii is an interesting candidate as microbial cell factory due to a striking resistance against short chain fatty acids – again pointing to the natural environment, where it thrives.
Microbial ecosystems biology as starting point for metabolic engineering
A microbial ecosystem can be defined as a system that consists of all the microorganisms that live in a certain area or niche and that function together in the context of the other biotic and abiotic factors of that niche .
The rumen is a fine example of such an ecosystem, functioning together with the ruminant, that provides the bioreactor (the rumen) and the pretreated carbon source (plant material), and receives in turn carbon and energy in a suitable chemical form. Overall the system can be viewed as one “super metabolism” which is distributed over a variety of organisms and species. The concept here is that particular metabolic pathways are distributed over different organsims. A changing environment, will lead to a change of that metabolism – on one hand by classical mechanisms, such as control of enzymatic activities or transcriptional control. On the other hand a change in community composition (relative numbers or even addition or omission of species) adds another possible layer of regulation.
As a concept this is quite appealing for a biorefinery. Instead of constructing one organism, which converts the carbon source directly into a useful product, one can think of the construction of microbial communities. Some strains enable the decomposition/deploymerisation of different feed stocks. Some strains detoxify the feed stock. Other microorganisms produce the chemicals of interest. Depending on the feed stock and depending on the desired product, the community can be composed in a different way. In other words: metabolic pathways are confined to individual species. By combining the right species - that is the right pathways - the metabolism can be changed - without cloning.
However, at the time being we are far from a detailed understanding of the ongoing in the rumen. While a wealth of new systems biological methods is becoming available  we still need to refine our conceptual basis to translate species- and community-data into a framework accounting for the interactions between metabolic networks of species in community enviroments. The rational de-novo construction of an artificial microbial ecosystem is one further step ahead.
However, the key message here is that understanding nature opens our view to exploit the wealth of possibilites in the future.
The authors gratefully acknowledge Giuseppe Rizzo for drawing the rumen and the cow for Figure 1. The authors’ research relating to adipic acid production and Megasphaera elsdenii is financially supported by the Translational Research Program of FWF Austria, project L391. The authors’ research relating to the use of lignocellulosic biomass for biorefinery applications is financially supported by the program “Intelligente Produktion”of FFG Austria, project “Lignoraffinerie”.
- Hungate RE: The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950, 14: 1-49.Google Scholar
- Sauer M, Mattanovich D: Construction of microbial cell factories for industrial bioprocesses. J Chem Technol Biotechnol. 2012, 87: 445-450. 10.1002/jctb.3711.View ArticleGoogle Scholar
- Wilson DB: Microbial diversity of cellulose hydrolysis. Curr Opin Microbiol. 2011, 14: 259-263. 10.1016/j.mib.2011.04.004View ArticleGoogle Scholar
- Weimer PJ, Russell JB, Muck RE: Lessons from the cow: what the ruminant animal can teach us about consolidated bioprocessing of cellulosic biomass. Bioresour Technol. 2009. 10.: 5323-5331.View ArticleGoogle Scholar
- Cheng KJ, Lee SS, Bae HD, Ha JK: Industrial Application of Rumen Microbes. Asian-Australian Journal of Animal Sciences. 1999, 12: 84-92.View ArticleGoogle Scholar
- Zheng Z, Chen T, Zhao M, Wang Z, Zhao X: Engineering Escherichia coli for succinate production from hemicellulose via consolidated bioprocessing. Microb Cell Fact. 2012, 11: 37- 10.1186/1475-2859-11-37View ArticleGoogle Scholar
- Leng RA, Steel JW, Luick JR: Contribution of propionate to glucose synthesis in sheep. Biochem J. 1967, 103: 785-790.View ArticleGoogle Scholar
- Sauer M, Porro D, Mattanovich D, Branduardi P: Microbial production of organic acids: expanding the markets. Trends Biotechnol. 2008, 26. 10.-108.View ArticleGoogle Scholar
- Zou W, Zhu LW, Li HM, Tang YJ: Significance of CO2 donor on the production of succinic acid by Actinobacillus succinogenes ATCC 55618. Microb Cell Fact. 2011. 10. 87.View ArticleGoogle Scholar
- Elsden SR, Gilchrist FM, Lewis D, Volcani BE: The formation of fatty acids by a Gram-negative coccus. Biochem J. 1951, 49: lxix-lxx.Google Scholar
- Marx H, Graf AB, Tatto NE, Thallinger GG, Mattanovich D, Sauer M: Genome sequence of the ruminal bacterium Megasphaera elsdenii. J Bacteriol. 2011, 193: 5578-5579. 10.1128/JB.05861-11View ArticleGoogle Scholar
- Raes J, Bork P: Molecular eco-systems biology: towards an understanding of community function. Nat Rev Microbiol. 2008, 6: 693-699. 10.1038/nrmicro1935View ArticleGoogle Scholar
- Röling WF, Ferrer M, Golyshin PN: Systems approaches to microbial communities and their functioning. Curr Opin Biotechnol. 2010, 21: 532-538. 10.1016/j.copbio.2010.06.007View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.